The Study Of Inflammation Inhibition By Polysaccharide From Ginkgo Biloba Leave

Inflammation is a complicated process. It is a protective reaction of the living organisms within blood vessel system to damaging factors. Moderate inflammation reaction is a self-protective process, while extreme inflammation reaction can result in immunity damage of the organisms and diseases. Although there are some anti-inflammation medicines, they cannot inhibit the serious inflammation. Therefore, it is more important to develop powerful anti-inflammation medicinesCell adhesion is an important physiological phenomenon for organism. Leukocyte adhesion to endothelium cell was found very important to preserve the organism normal activity, induce inflammation, immunity and the tumor metastasis. Whether this adhesion can be blocked is crucial to developing new anti-inflammation medicine. Hence, studying leukocyte and endothelium cell adhesion can help us understand the mechanism of the interrelated diseases and control them. At present, anti-adhesion has become a new method to cure diseases, and the anti-adhesion of polysaccharide also be widely noticed.We extracted PGBL from Ginkgo biloba leaf. It can eliminate oxygen, -OH and it also can anti-senescence, inhibit mice immunity cell and so on. So it has higher values and can be widely developed.This experiment was performed by purification of the PGBL, mice acute peritonitis in vivo and flow chamber to study its anti-inflammation activity and mechanism. The result showed that 25mg/kg and 50mg/kg dose PGBL has strong inhibition to mice acute peritonitis. At concentrations of 0.1~0.5g/L PGBL can inhibit the adhesion of neutrophils and CHO cells expressing P-selectin and shows the anti-inflammation molecule mechanism of PGBL is blocking the interaction of P-selectin binding to its ligand and inhibiting neutrophils rolling, adhesion and infiltration to vascular endothelial cell.