Modified Heparin Can Inhibit P-selectin-mediated Adhesion Of Leucocytes

Inflammation is that the live tissues with blood vessel react to stimulus in order to protect themselves. Moderate inflammation is good to organism and can protect itself, while excess inflammation can damage to the organism and result in many symptoms. Although there are some anti-inflammation medicines, they cannot inhibit this severe inflammation. Therefore developing one kind of powerful anti-inflammation medicine is more and more important. The adhesion of the leucytoes is the feature for inflammation.The moleular basis lies in the interaction of leucytoes to the adhesion moleculars on entholium. The selectins are a family of intercellular adhesion molecules that mediate the initial adhesion of leukocytes to the endothelia of blood vessels during inflammation. Whether this adhesion can be pathologically blocked is crucial to developing new anti-inflammation medicine. It is reported that heparin is not only one kind of anticoagulant but also can be used to inhibit inflammation. But it can result in bleeding, which hold back its use in anti-inflammation.In order to induce the anticoagulation of heparin and emphasize the anti-inflammation, we modified heparin with many chemical methods such as sulfatation, desulfatation, oxidation, deoxidization and so on. We got six kinds of heparin derivatives, which are RO-heparin, CRS-heparin, 2/3ODS-heparin N/2/3DS-heparin, NDS-heparin, and NDS/NAc-heparin. We tested the change of structure in heparin and chemically modified heparin with CL-6B chromatography. It is tested that the structure of them is complete. And another experiment of APTT makes us be sure that the modified heparin has been deduced. Then in the adhesion of P-Selectin with leucocytes, we found that heparin and modified heparin can inhibit this adhesion. All these attest that heparin and modified heparin can inhibit the adhesion of leucocytes. Now we have founded thioglycollat-induced peritonitis animal model and the experiments in vivo is being done. On the basis of these recent experiments, itcan be tested that heparin and modified heparin can inhibit the adhesion of leucocytes. As modified heparin is very easy to be got and is safe for avoiding bleeding, it can be developed a new kind of anti-inflammation medicine.