Elemene And Xiaoaiping Induces Apoptosis And Regulates Expression Of Bcl-2 Protein In Cervix Cancer Hela Cells

IntroductionThe first is Elemene from the AMRS Zingiberaceae temperature Ezhu (Curcum a aromatica Salisb) extracted an effective anticancer ingredients. Nearly 30 years of basic research and clinical application confirmed that elemene with a broad spectrum of anti-tumor therapy, toxicity characteristics. Now widely used for various clinical tumor therapy. According to XiaoAiPing is cancer of bone League in the sterile water solvent, clinic for advanced gastric cancer and liver artery interventional therapy achieved satisfactory results. Hela cell is a pair of drugs and the environment than a cell tolerance, in the treatment of cervical cancer is also difficult clinical, The study Elemene and XiaoAiPing cervical carcinoma cell line Hela mechanism and the role to explore review with a view to the adoption of the two kinds of medicine in vitro inhibitory effect of Chinese medicine to reveal the role of anti-tumor mechanism and the similarities and differences between the two , and grandfather magazine reported in sensitizer, induces apoptosis and reduced protein expression in line and many other mechanisms, turn to sum up all the mechanisms play a leading role in what kind. The study is only the beginning of a series of studies.PurposeElemene and Xiaoaiping Induces Apoptosis and Regulates Expression of Bcl-2 Protein in Cervix Cancer Hela Cells Materials and methodsThe cell proliferation assay for the growing number of Hela cells were seeded in 96-well culture plates, different concentrations of XiaoAiPing and the consumption level, so a final concentration of 0, 25, 50, 100, 200, 400 ml, the final use of enzyme-linked detector wavelength of 570 nm was measured absorbance A value and evaluates the IC50 value. Were detected by flow cytometry and cell cycle and apoptosis detection gel electrophoresis gradient and the collection of different concentrations Elemene and XiaoAiPing the role of cross-Hela cells using electron microscopy observation of cell morphology and acridine orange staining and cell chromatin RNA changes and changes in the ultrastructure of cells. Bcl-2 protein levels in the collection of different treatment groups for 48 h in Hela cells, RIPA buffer admission to the supernatant after using BCA protein concentration measured after 2 h at room temperature hybridization. After washing 454th film to labered rabbit anti-goat antibodies (1:5000), a hybrid h at room temperature. 454th washing after ECL membrane color. Statistical samples were compared using Dunnett's test.ResultsElemene, eliminate cancer-Hela cells in vitro : Elemene and XiaoAiPing 25-400 g ml for 72 h on Hela cells in vitro significantly inhibited , in a dose-dependent manner. Flow cytometry analysis Elemene and XiaoAiPing two combination of apoptosis was significantly better than the two drugs alone. When the role of 6 hours, the cells in G1 phase decreased slightly, S phase cells gradually increased. 12 h, the cells in S phase arrest. 24 h, the G1 phase were significantly increased, apparently hypodiploid peak, the control group unnecessary features. Use of inverted microscope observation of cell morphology can be seen for the drug cell shrinkage, smaller, and the control group uniform cell size; AO staining cells drugs chromatin condensation, the rupture, there intracellular nuclear-like structure cytoplasm lighter color, Nuclear control group of yellow-green, large, orange-red cytoplasm; Electron microscopy showed typical crescent nuclei of apoptotic cells. and apoptotic cell membrane integrity of apoptotic bodies.Bcl-2 protein detection : 100 g /ml Elemene and XiaoAiPing the two combined for 72 h, 1% agarose gel electrophoresis was clear apoptosis characteristic trapezoidal strip.ConclusionThis study used different concentrations - Elemene and XiaoAiPing the two combination in Hela cells, found elemene cancer and the consumption level of Hela cells in vitro significantly, having synergies. Elemene cancer and the average consumption significantly induced apoptosis induced apoptosis was concentration and time dependent, Note elemene cancer and the average consumer can induce apoptosis, play its role in cancer. Bcl-2 protein expression detection analysis showed that In Elemene and XiaoAiPing tumor cells in the induction of apoptosis are accompanied with the expression of Bcl-2 protein levels dropped, Therefore, Bcl-2 reduced the Elemene and XiaoAiPing Hela-induced apoptosis process is a vital -. Suggest Elemene and XiaoAiPing induced apoptosis in the process of adoption is likely to have some effect on the way bcl-2 gene so that it inhibited the expression, initiate or promote cell apoptosis. But its anti-tumor mechanism of the target and the associated signaling pathways and other issues have not yet revealed Also pending further in-depth and meticulous research. Meanwhile, elemene and joint consumer-cancer role in the induction of apoptosis Hela was better than the two drugs alone. accompanied by a level of Bcl-2 protein expression in the years ahead. Suggest elemene and consumers may have cancer - synergy of Chinese medicine anti-cancer drug in combination with an optimistic outlook. But elemene and consumer anti-tumor cancer of the main clinical indications and applications of different tumor dose of the best, collaborative application the best time and best compatibility programs require further research to better applied to clinical oncology.