Experimental Study Of Rabbit Radial Bone Defects Repaired By Autogenous Bone Marrow Mesenchymal Stem Cells Carrier Complex

Experimental study of rabbit radial bone defects repaired by autogenous bone marrow mesenchymal stem cells carrier complexPrefaceThe repair of bone defects in plastic and orthopedic surgery continues to pose a number of clinical problems. Although autologous bone has been the historical standard for these procedures as a source of reconstructive material, it has significant limitations. In particular, donor site morbidity, including pain, loss of function, and local injury in the harvesting procedure, and a limited supply of bone are among the most significant of these. The use of allograft also carries with it significant problems, including disease transmission, host rejection, and lack of osteoinductive properties.The surgical reconstruction of any tissue will require reparative cells and the appropriate scaffold to both introduce the cells into the wound site and support tissue specific biosynthetic events. Additionally, inductive and phenotype-specific growth factors and cytokines must sequentially interact with the implanted cells and their progeny to effectively consummate a tissue engineered reformation of functional tissue.The research of bone tissue engineering is that one difficult problem resolving this has opened up a frontier, At present, a lot of scholar research this. but has almost pay attention to extracorporeal cultivated conditional research, but has ignored to investigation and discussion improving local microenvironment.ObjectiveTry to use this experiment prepare containing autogenous bone marrow mesenchymal stem cells and demineralized bone matrix (DBM) carrier compound scaffold material embedding in the bone defect position, simultaneous, to implantation basic fibroblast growth factor (bFGF)and vitamin C(VitC) in microenvironment, strengthen bone repair ability and improve the effect of the repair. MethodsChoose 30 New Zealand White rabbit (3-month-old), bone marrow was obtained from left ilium and femur tuberosity of all rabbits. MSCs are purified with the density gradient centrifugation, culture-expanded in vitro, and combination with DBM carrier compound scaffold material, The mixture was used respectively to repair 10 mm segmental defects created in the radius of rabbits. Experimental side(the left radius defect, A, n=24) was rapaired by MSCs, DBM, calcium alginate(CA),bFGF and VitC. Experimental-Control side(the right radius defect, B, n=24) was repaired by MSCs,DBM and CA. Control side (C, n=3) was repaired by DBM and CA. Blank group(D, n=3) was not filled with anything. Every 10 rabbits were sacrificed in the 30,60 and 90 days postoperatively, the rapair capability was assessed by physically,radiographically,histologically and immunohistochemicaly.ResultsRoentgenographically, the bone defects that had been treated with grafts exhibited new bone formation increased with time. But the result of A group's were apparently superior to that of the B group's,C group's at 30,60,90 days after operation ( P<0.05). The rate and quality of new bone formation were significantly different in the experimental and the control groups on histologic analysis (P<0.05). A group's callus and transplanting transformation bone are obvious quicker than B group's and C group's; DBM cartier compound scaffold material quicker than B group's and C group's, the control group's scaffold residue obvious exceed experimental group's. The bone defects in experimental group were repaired and the new bone formed in multipoint way, and osteoid tissue, cartilage, woven bone and lamellar bone occurred earlier when compared with control group in which the bone defects were repaired in "creep substitution" way. The bone defects in D group's remained same size at the 90 days.ConclusionsAdding bFGF and VitC—beneficial to bone repair factor—in microenvironment, when embedding extracorporeal culture transplante can improve the effect of bone defect repair.