The Effect Of Lactobacillus On Intestinal Permeability And The Clearance Of Middle/small Molecule Substance

Objective: To investigate the effect of intestinal microflora on intestinal permeability and middle/small molecular substance.Methods: we divided forty 5-to-6-week-male Spragua-Dawley rats of about 200g-weighted into four groups as pseudo operation group, 5/6 nephrotomized model group, bifidobacterium eaten group and lactobacillus eaten group. The pseudo operation group 5/6 nephrotomized model group were raised with bifidobacterium and lactobacillus. Seven days later, take the blood and stool sample, centrifugate the blood sample to obtain plasma, dilute the stool sample with distilled water, take the supernatant after mingling even, and detect the middle molecular substance by infrared absorption and the number of OD were recorded at 254nm,and the number of OD represents the content of middle molecular substance. Take the other blood and stool samples, detect the blood urea nitrogen and creatinine, then calculate the blood/stool ratio of them.Results: 1) In the pseudo operation group, data showed no significant difference in middle molecular substance blood urea nitrogen and creatinine before and after treatment in blood and stool(P>0.05). 2) Compared with the pseudo operation model group, the contents of middle molecular substance blood urea nitrogen and creatinine in the blood and stool increased remarkably (P<0.05), and the D-lactic acid in the blood increased (P<0.05). 3) Compared with the pseudo operation model group, the content of middle molecular substance in the blood of bifidobacterium treated group and the group treated with lactobacillus (P<0.05); in the stool sample, the content of middle molecular substance in the group treated with bifidobacterium increased (P<0.05), while it decreased in the group treated with lactobacillus (P<0.05). There was no obvious difference in the blood/stool ratio of middle molecular substance blood urea nitrogen and creatinine. The D-lactic acid in the blood of the group treated with bifidobacterium and with lactobacillus, compared with the. 5/6 nephrotomized model group, decreased remarkably (P<0.05). 4) Compared with the psuedo operation group, there were no difference in middle molecular substance blood urea nitrogen and creatinine both of the group treated with bifidobacterium and with lactobacillus in the blood and the stool sample (P>0.05), and it showed no difference in D-lactic acid in the blood (P<0.05). 5) It's negative correlation between the contain of D-lactic acid middle molecular substance BUN, Cr and the numbers of bifidobacterium lactobacillus.Conclusions: 1. The D-lactic acid in the blood in the rats with chronic renal failure increased, which suggested that the intestinal permeability increased.2. Both of bifidobacterium and lactobacillus can regulate the intestinal microflora of rats with chronic renal failure, recover the intestinal permeability3.It can promote the intestinal to clear middle molecular substance and decrease the content in blood to feed the chonic renal failure rats with bifidobacterium or lactobacillus through the enteron.4. Bifidobacterium and lactobacillus can decrease the content of blood urea nitrogen and creatinine in blood by decomposing urea and creatinine in gastrointestinal tract.