Expression Analysis Of Gene Mcpr1 During Murine Palatin Development And Cleft Palate

mcpr1 was a novel gene cloned by subtractive hybridization method at our development, which was obtained by comparing palatal shelves of normal murine embryo with ones of cleft palate models induced by retinoic acid. It was registered in GenBank as AYO74887, and named mouse cleft palate related gene. mRNA length of mcpr1 gene had been identified, and expressions in some tissues had been observed. This study includes two parts involved in revealing functions of mcpr1 gene in embryonic palatin development. So is the following.1.Expression analysis of gene mcpr1 during palatin development and cleft palateRT-PCR and immunohistochemistry analysis showed that mcpr1 expressed stronger in murine palate induced by retinoic acid than normal palate.2. The effect of mcpr1 on biological characterization of MEPM cellsRT-PCR and immunohistochemistry analysis showed that mcpr1 expressed stronger in MEPM cells induced by retinoic acid than normal palate. It might infer that mcpr1 was one of the down-stream regulation genes of RA.EPM cells were induced by fusion protein GST-mcpr1 with different density, and the results of MTT showed that GST-mcpr1 might inhibit the cellular growth, moreover, the inhibition effect was decided by the density of GST-mcpr1; It was observed that the fusion protein can contribute to cellular apoptosis by TUNEL method.It's necessary to do lots of experiments to discover the function of the novel gene. This article showed that mcpr1 was one of the down-stream regulation genes of RA and might play an important role by being involved in PCD during the embryonic palatin development. it is the key of discovering the function of mcpr1 to investigate the regulation mechanism of post-transcription and post-translation.