| Objective: Many Researches show that, different gene polymorphisms of Lipoprotein lipase(LPL) have certain effects on blood lipids (especially on triglyceride , TG), while hypertriglyceridemia(HTG) is an important risk factor for atherosclerotic cerebral infarction(ACI). The aim of this study is to investigate the association of PvuⅡand N291S polymorphisms in LPL gene with HTG and ACI.Methods: The genotype and allele frequencies of LPL gene polymorphisms (PvuⅡand N291S) were analyzed by PCR-restriction fragment length polymorphism in 41 ACI patients and 88 random-selected healthy control individuals. Meanwhile serum triglyceride levels were determined. The correlation of high blood pressure, diabetes, high cholesterol, smoking, body mass index, PvuⅡand N291S gene polymorphisms with the incidence of ACI were also analyzed.Results1 The general state of ACI group and the control group: In two groups there was no difference between the age and sex ratio. ACI group of patients have higher body mass index without statistical significance (P>0.05), but Significantly higher smoking rates, prevalence of hypertension and diabetes compared with the control group (P<0.05).2 In the two groups, P+P- genotype were prevalent, followed by P+P+ and P-P- is relatively rare; P+ is the main allele and P- is the rare allele. The genotype distribution of PvuⅡin both groups corresponds to Hardy-Weinberg equilibrium, with groups'representation. In ACI group and the control group, P+P+,P+P-,P-P- genotype frequencies are 0.341vs0.295, 0.512vs0.534, 0.147vs0.171 respectively, without statistical significance (P>0.05); P+,P- allele frequencies are 0.598vs0.563, 0.402vs0.437 respectively, the differences between the two groups were also without statistically significant (P>0.05).3 In ACI group, serum triglyceride concentrations of P+P+,P+P-,P-P- genotype are 3.50±2.32vs2.75±2.07vs1.88±1.04 respectively, exhibiting a gradual decrease, but without statistical significance(F=1.44,P=0.2504). In control group, serum triglyceride concentrations of P+P+,P+P-,P-P- genotype are 2.07±0.61vs1.88±0.57vs1.87±0.57 respectively, also without statistically significant differences among the three(F=1.01,P= 0.3672).4 In HTG and NTG subgroups of ACI group, P+P+,P+P-,P-P- genotype frequencies are 0.370vs0.286, 0.556vs0.429, 0.074vs0.285 respectively, without statistical significance (X2= 0.038,P= 0.846>0.05). P+,P- allele frequencies are 0.648vs 0.500, 0.352vs0.500 respectively, without statistical significance (P>0.05). In HTG and NTG subgroups of the controls, P+P+,P+P-,P-P- genotype frequencies are 0.224vs0.385, 0.571vs 0.487, 0.115vs0.128 respectively, without statistical significance (X2=2.907,P=0.234>0.05). P+,P- allele frequencies are 0.510 vs0.628, 0.490vs0.372, without statistical significance(P>0.05).5 A multivariate logistic regression analysis, with a =0.1 as the selective and exclusive criterion. The dependent variable was getting the ACI or not and the independent variables included hypertension, diabetes, smoking, carrying P+ allele, obesity(BMI) and other risk factors for ACI. It is found that smoking history, hypertension, diabetes were brought into the model, as independent risk factors for ACI; while carrying P+ allele was not.6 N291S mutation of LPL was not found in either group.Conclusion1 The allele P+ had no significant correlation with serum triglyceride levels.2 The allele P+, which had no association with ACI, couldn't be regarded as one of the independent genetic risk factors for ACI in Chinese population.3 N291S mutation of LPL, which had certain association with HTG and ACI in European Whites, was not found in either group. Maybe it is not associated with Chinese incidence of HTG and ACI.4 History of smoking, hypertension and diabetes were independent risk factors for ACI respectively, which is in line with the understanding of the past.5 The study found no direct correlation between obesity and the incidence of ACI.
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