| Objective To investigate the prevalence rates and its related factors of BacterialVaginosis(BV) and other STDs in Venereal Disease clinic and obstetrics &gynecology clinic in Tianjin. To find an effective method for isolation andidentification of Gardnerella vaginalis(GV). To determine the minimal inhibitoryconcentration(MIC) and to identify the association of biotypes,genotypes of GVwith BV. Methods 546 women patients were involved in the study of BV and otherSTDs' pathogens(including Chlamydia trachomatis, Ureaplasma urealyticum,Neisseria gonorrhea, Candida monilia, Trichomonas vaginalis, syphilis, HIV,Condyloma acuminatum)were detected. GV were inoculated and cultivated by home-made HBT plate. Biochemical identification was taken based on four simpleprocedures(including colonial morphology, clear beta-hemolysis with diffuse edges onHBT plate, negative catalase test and the typical cell morphology in the Gram stainedsmear) and complicated procedure respectively. The semiquantitation of number ofcolonies of GV was studied. The susceptibility test in vitro of some antimicrobialagents(including Metronidazole,Clindamycin,Ciprofloxacin,Roxithromycin,,Tetracycline,Cefuroxime and Ampicillin)were analysed. GV were typed intodifferent biotypes on the basis of hippurate hydrolysis,β-galactosidase and lipaseactivity of Briselden's method and typed into different genotypes byARDRA(Amplified ribosomal DNA restriction analysis). The association betweenbiotypes, genotypes and BV were analysed. Results The prevalent rates of BV inpatients of Venereal Disease clinic and obstetrics & gynecology clinic were 27.2% and22.0% respectively, which had no statistical difference (P＞0.05). The mixed infectionwas 63.4%. Ureaplasma urealyticum was the most common pathogen in mixedinfection. Abortion history, applying intrauterine contraceptive device and multiplesexual partners had the association with BV. There was no statistically differencebetween the two identification methods (x~2=3.2, P＞0.05). Among 129 strains of GV,the isolating rates of GV in patients with BV (48.1%) was higher than that of patientswithout BV(15.9%), which had obviously statistical difference (x~2=57.17,P＜0.001). The isolation of 4+ growth of GV from BV patients was higher than thatfrom patients without BV, while, 1+,2+ growth of GV was lower(P＜0.05). Thesusceptible agents were: Clindamycin,Roxithromycin,Tetracycline andMetronidazole. In 128 women diagnosed as BV the prevalent rate was 24.1%; Theisolating rates of GV in patients with BV (48.4%) was higher than that of patientswithout BV(13.1%), which had obviously statistical difference (P＜0.001). Sevenbiotypes were found in 115 strains of GV. Biotype 2 (24.4%)was the most frequentone, followed by biotype 3(21.7%) and biotype 5(18.3%). GV biotype 1,2,3,and 4were found more in patients with BV than those without BV (P＜0.001). Biotype 5 was seen more frequently among women without BV(P＜0.05). Biotype 6 was not observed.There was no statistical difference in distribution of biotype 7 and 8.50 strains of GVwere genotyped into three kinds(genotypeA,B and C). There was no association ofgenotyping with BV. Conelusions BV was not only one of common diseases inobstetrics and gynecology clinic, but also related to sexually transmited disease. Weshould develop Screening work in high risk group because of high morbility andfrequently mixing infection. HBT plate was a sensitive medium for the isolation ofGV, but was also very accurate for its presumptive identification. GV was one ofpathogenic microbes of BV. Clindamycin was found to be the most susceptible agentsfor GV. Biotypes of GV were associated with BV, whereas genotypes of GV were notfound to have association with BV.
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