Study Of The Protective Effects And Mechanism On Alcoholic Hepatic Injury Of The Compound Codonopsis Lanceolata

With the development of age, the alcohol consumption has been rushed in recentyears. It is reported that the incidence rate of hepatic injury induced by alcohol (ALD)is higher than that of some other hepatic disease and to which more attention are paidspontaneously.Codonopsis lanceolata (CL) is a kind of medical herbal roots (now used as a kindof food), composed of many kinds of nutrient substances. In order to explore theprotective effect and its primitive mechanism of Codonopsis lanceolata on alcoholichepatic injury, we have designed this series of trails. According the status of bodyweight, the 40 Kunming mice were randomly divided into four groups with 10 micein each group: normal control group, alcoholic group, low—dosage group (1g/kg) andhigh—dosage group. Mice in the normal group and alcoholic group were treated withintragastric administration of distilled water in the morning, the low—dosage andhigh—dosage groups were given intragastric administration of 1 and 2 g/kgCodonopsis lanceolata extract respectively. In the afternoon, only mice in the normalcontrol group were treated with intragastric administration of distilled water, those inthe other 3 groups were given intragastric administration of edible alcohol(450mL/L). After 8 weeks, the content of TG, MDA were detectived. It was foundthat drugs could significantly reduce the content of hepatic TG and MDA, and as faras those effects were concerned. the high—dosage group was better than the low—dosage group. Thus. we choose the high—dosage drug to investigate protectiveeffects on alcoholic hepatic injury of the compound Codonopsis lanceolata.In the protective experiment, 50 mice were randomly divided into five groupswith 10 mice in each group: normal control group, alcoholic group, Codonopsislanceolata extract group, Semen Cassiae extract group, compound Codonopsis lanceolata extract group (Codonopsis lanceolata extract+Semen Cassiae extract).Mice in the normal group and alcoholic group were treated with intragastricadministration of distilled water in the morning, And the three drug groups were givenintragastric administration of Codonopsis lanceolata extract (2g/kg), Semen Cassiaeextract(1g/kg) and the combination of them[(2g Codonopsis lanceolata extract+1gSemen Cassiae extract)/kg[ respectively. In the afternoon, only mice in the normalcontrol group were treated with intragastric administration of distilled water, those inthe other 4 grouPs were given intragastric administration of edible alcohol(450mL/L). After 8 weeks, the content of TG, MDA were detected and liverhistology was also examined. Compared with alcoholic group, drugs couldsignificantly reduce the content of hepatic TG and MDA. Changes of liver histologyimproved obviously in the drug groups as compared with the alcoholic group.To study the mechanisms intensively, The activity of hepatic SOD and GSH-Pxand the content of hepatic Se were detected. The result indicated that drugsremarkably elevated the hepatic SOD,GSH-Px activity and the content of hepaticSe. And the effect of compound Codonopsis lanceolata is best. Following. we alsodetectived the content of Se in the Codonopsis lanceolata extract and Semen Cassiaeextract, The result indicated that the content of Se is 5.508ng/g in the Codonopsislanceolata extract and 4.471 ng/g in the Semen Cassiae extract. In the conclusion,Semen Cassiae and Codonopsis lanceolata have some effects on the loss of elementsin the liver tissue as well as the inhibition of the LPO generation, and may be add Seto the body.