The Studies Of Cultured Cordyceps Militaris On Regulating Blood Lipid

With the improvement of life, cardiovascular disease,adiposity,hypertension and diabetes have became the main diseases harmful tohealth, and the cardiovascular disease was listed as the top risk to deathin China. The pathogeny for the cardiovascular disease is complicated, butthe abnormity of lipid metabolism plays a key role. So, how to regulateblood lipid effectively is important to prevent cardiovascular diseaseat present. Mushrooms have attracted the attention of researchers recentyears due to their tonic and pharmacological functions. Cultured Cordycepsmilitaris have been found to have pharmacological effects similar to thatof the Cordyceps sinensis. They not only account for the immunomodulatory,antibacterial,antitumor,anti-inflammatory,hypoglycaemic,hypolipidaemic effects, but also have been used as healthy foods withoutside effect. Cultured Cordyceps militaris which could regulate bloodlipid has great potential in the field of healthy food in the future.Based on our preious study, experiments were carried out toinvestigate the effects of mycelium and fruit bodying of culturedCordyceps militaris on regulating blood lipid using mice as experimentalmodel and persons as clinical observation. The main procedures and resultswere shown as follows:1. The effect of the mycelia of cultured Cordyceps militaris onregulation of blood lipid in mice with high blood lipid: one hundred andthirty-five KM mice were randomly assigned to nine groups (n=15 in eachgroup):base control group, high lipid model group and positive controlgroup, the dosage of 0.14, 0.28, 0.56g/kg bw/day mycelia of Cordycepsmilitaris which is pre-heated or not. While the animals of base controlgroup were received only distilled water by garage, the others were treatedwith high lipid emulsion 0.5mL/day/animal in the morning and different samples(l.mL/day/animal) in the afternoon by garage and total for 21days. The body weigh of mice were weighed every 6 days. The blood wasextracted from the tails and the total cholesterol(TC) of serum wasmeasured on the Oth day, 7th day and 14th day. On the 2th day, the mice weredissected after taking out the blood from the mice's eyeballs. The TC,triglyeride(TG),high-density lipoprotein cholesterol(HDL-C) ofserum were examined and low-density lipoprotein cholesterol(LDL-C),atherogenic indices (AI),liver weigh indices et. al. were calculated Onthe 21th day. The results suggested that the serum TC,TG of high lipid micewhich were treated with mycelia of Cordyceps militaris (heated or not)andJin-shuibao(the positive control group) was significantly reducedcompared to high lipid control group(p＜0.05) on the 21th day. The liver weighindices of the groups treated with mycelia of Cordyceps militaris (heatedor not) extremely and significantly decreased in mice comparing to thehigh lipid model group(p＜0.01).2. The study of the fruiting body of cultured Cordyceps militaris onregulation of blood lipid in mice with high blood lipid. Ninety KM micewere randomly assigned to nine groups (n=10 in each group):base controlgroup, high lipid model group, positive control group, and groups treatedwith different fruiting body. Fresh fruiting body A group(treated every2d) and B group(treated every 3d) were treated with 13.34g/kg bw freshfruiting body, heat dry fruiting body A group(treated every 2d) and Bgroup(treated every 3d) with 2.67g/kg bw dry fruiting body by heating andfreezing dry fruiting body A group(treated every 2d) and B group(treatedevery 3d)with 2.67g/kg bw fruiting body dry by freezing. While the animalsof base control group were received only distilled water by garage, theothers were treated with high lipid emulsion 0.5mL/animal in themorning, and were received of different samples 1mL/animal in theafternoon by gavage every two or three days and total for 45 days. The body weigh of mice were weighed on the 0th day,30th day and 45th day. The blood fromthe tails was extracted and the TC, TG of serum was measured on the 0th day,15th day and 30th day. On the 45th day, the mice were dissected after takingout the blood from the mice's eyeballs. Their serum TC, TG, HDL-C wereexamined and LDL-C, viscus weigh indices et. al, were calculated. It wasshown that except for the heat dry fruiting body B group, the other groupstreated with fruiting body significantly reduced the serum TC,TG in thehigh lipid mice comparing to the high lipid model group(p＜0.05, p＜0.01).Onthe 45th day, except for the heart weigh indices in mice, there are nosignificant differences between fruiting body groups and the high lipidmodel group for liver, spleen, lung, kidney weigh indices in mice.3. The clinical observation with the fruiting body of culturedCordyceps militaris on blood lipid regulation with high blood lipidpersons. Thirteen volunteers with high blood lipid were randomly choosenfrom the employees who had been retired from the South China NormalUniversity and were told to eat the fruiting body of cultured Cordycepsmilitaris dryed below 40℃with the dosage of 5g/3d/person for 3months. The record table was written down and the physical examination wasdone before and after experiment. The serum samples were analyzed by theGlamour-2000 automatic chemistry analyzer in the hospital of theuniversity. The change of blood biochemical indices and hematologicalparameters were analysed. The result showed that the average of serum TGin patients is significantly decreased(P＜0.05) from 2.39mmol/L beforeexperiment to 1.69mmol/L after the dry fruiting body of cultured Cordycepsmilitaris taking. But the serum TC, HDL-C, LDL-C in volunteers did not havesignificant differences after eating the dry fruiting body of culturedCordyceps militaris, similar to other blood biochemical indices andhematological parameters.Based on the result of the study, the serum TC,TG in high blood lipid mice were significantly reduced feeded with the mycelia and fruit bodyingof cultured Cordyceps militaris. The serum TG in high blood lipid personswere significantly reduced after taking dry fruit bodying of culturedCordyceps militaris with the dosage of 5g/3d/person. It is implied thatcultured Cordyceps militaris can regulate blood lipid and used as thehealthy food for a long time.