Extraction, Purification, Characterization And Antioxidative Activities Of Polysaccharides From Scutellaria Barbata D.Don

Scutellaria barbata D.Don is perennial herbaceous plant and belongs toScutellari of the family Labiatae. It has been used for medicines for a long timein China. In order to strengthen the comprehensive utilization of ScutellariaBarbata, Scutellaria Barbata polysaccharide's extraction, purification and theactivity of antioxidation were studied. The effect on extraction efficiency wasinvestigated by means of single factor and orthogonal experiments. SBP_W andSBP_S were gained by using Sephdex and DEAE column chromatography andSBPs's homogeneity was proved by fast protein liquid chromatography (FPLC)and PAGE, its compositon was studied by means of GC-MS. The activity ofantioxidation was studied. The results as follows:1 The parameter of extract technology was perfected; the optimal waterextraction parameters were as follows: tempreture 70℃, extracting 1 times,6hours, solid-liquid ratio 1:30. The yield of Scutellaria Barbata polysaccharidewas 2.703ï¼….2 SBP_W and SBP_S wre isolated and further purified by DEAE-Cellulose-52and Superdex 200 column chromatography. The IR of them (3420 (O-H),2940(C-H),1665 (C=O),1445 (C-O),1120 (C-O) cm^-1) showed characteristicabsorptions of polysaccharide.3 The content of total saccharide of SBP_W and SBP_S was 89.60ï¼…and 92.57ï¼…, and the acidic saccharide was 4.9ï¼…and 19.6ï¼…respectively. SBPs'shomogeneity was proved by fast protein liquid chromatography (FPLC) andPAGE. Its relative molecular weight was estimated to be 1.11×10⁴ by FPLC.GC-MS analysis indicated that it was composed of Rha, Fuc, Ara, Xyl, Man, Glc,and Gal with a molar ratio of 1.00:0.13:0.80:0.10:0.34:0.56:3.04.4 The antioxidant ability of SBP_W and SBP_S in vitro were studied. Theinhibitions of the SBPs to O₂^-·and·OH were assayed by the method ofpyrogallol and the Fenton system. SBP_W had active ability in scavenging thesuperoxide anion radical and hydroxyl free radical, the IC₅₀ value was 921.73μg/mL and 89.26μg/mL, and SBPs also had active ability in scavenging the superoxide anion radical and hydroxyl free radical, the IC₅₀ value was 800.00μg/mL and 90.42μg/mL respectively. The results indicated that SBP_W and SBP_Shad scavenging effects on hydroxyl radical and superoxide anion radical, hadthe inhibiting effects on lipid peroxidation induced by Fe²⁺ and H₂O₂, inhibit thehemolysis of red blood cells. In different antioxidant systems, SBP_W and SBP_Shad different antioxidant ability.5 Aged mice (nine months) were abdominal administrated with SBP_W andSBP_S at dosages of 100～400mg·kg^-1 for 35 days. SBP_W and SBP_S couldobviously decrease the content of serum and liver lipoperoxide (MDA), and alsoincrease the activity of superoxide dismutase (SOD) of serum and liver in agedmice. The results showed that SBP_W and SBP_S have an antioxidative effect,suggesting that it has antiageingfunction.