Experimental Study On Preparation And Its Bioactivities Of Inulin And Oligochitosan's Sulfate

The inulin, a soluble meal fiber, has many kinds of physiological functions, suchas improving the balance of friendly bacteria in the bowel, promoting the absorptionof the calcium, declining blood sugar and proventing fat, and so on. At present, manypeople have been paid to study on the carboxymethylation of inulin. But few reportson sulfation of inulin were published, and so, inulin sulfate was obtained by using ofsulfate modification method at the basis of our lab's conditions. The aim is to expandthe bioactivities and to expand the applications of inulin.Oligochitosan also has many kinds of physiological functions, such as antifungal,antitumor, immunostimulating effects, and so on. By now, some researchers havereported anticoagulant activity of chitosan polysulfate derivatives, heterochitosansand their oligosaccharide sulfates, however, few reports on sulfation oflow-molecular-weight (Mv＜1000 Da, DP:2～10) were published, and so,oligochitosan sulfate was obtained by using of sulfate modification method at thebasis of our lab's conditions. The aim is to test the change of the lower molecularweight oligochitosan's bioactivities.In this study, inulin sulfate and oligochitosan sulfate were obtained by using ofsulfate modification method with chlorosulfonic acid-pyridene. And mainly studiedsulfation-techniques, physicochemical properties and biological activities of the inulinsulfate and the oligochitosan sulfate. The samples were treated through SephadexG-25 column to purify, and sulfur content(S%)of the samples were measured byturbidimetrically asbarium sulfate. The structure and substitution pattern were studiedusing UV, FT-IR and NMR spectroscopy. The results showed that the conformationand other physicochemical properties of inulin sulfate were changed by sulfate radicaland the degree of substitution(DS)of which were determined to be 0.45, 0.98, 1.19and 1.71. As was shown by FT-IR and NMR spectroscopy, the sulfation mainlyoccurred at C-6 and C-4 positions of fructose and C-6 and C-4 positions of glucose;secondly, occurred at C-3 position of fructose and C-2 position of glucose. The degreeof substitution of oligochitosan sulfate was determined to be 0.22, 0.77, 1.23 and 1.50and the sulfation occurred at C-6, C-2 and C-3 positions. With regard to theirbiological activities, we studied the antifungal activity, induced resistance activity andanticoagulant activity of inulin and its derivates. Simultaneity, we also studied theanti- Phytophthora capsici activity, induced resistance activity and anticoagulant activity of oligochitosan and its derivates. The results showed that inulin was notshown to have anti-fungus activity, induced resistance activity and anticoagulantactivity, but inulin sulfate was shown to have a certain anti-fungus activity, inducedresistance activity and strong anticoagulant activity. About the bioactivity ofoligochitosan, such as anti- Phytophthora capsici activity, induced resistance activitywere disappeared, and the oligochitosan and its sulfate were not shown to haveanticoagulant activity.