Regulating Role And Clinical Meaning Of TSP-1 And VEGF In Microvascularization Of Gastric Cancer

Objective : Regulating Role and clinical meaning of TSP-1 and VEGF in microvascularization of gastric cancer.Methods: A total of 85 primary gastric cancer and its normal tissue specimens were obtained from gastric cancer patients undergoing surgical therapy for the treatment of gastric cancer. Each tissue we got five section. 85 cases of gastric carcinoma and its normal tissue were studied on the expression of TSP-1, VEGF protein and microvessel density with SP immunohistochemical technique to evaluate the relation of the expressions of TSP-1, VEGF with microvessel density and parameter of clinical pathology. Results: (1) The signals of all tissue section were in focus. (2).34 cases for TSP-1(40%) and 69 cases for VEGF (81.2%) protein were positive respectively and the average MVD was 28.17±3.96 vessel per 200 field of vision in the gastric carcinoma group. Most positive staining for TSP-1 and VEGF was seen in the cytoplasm of the cancer cells, a few was in the extracellular matrix. (3) The rate of TSP-1 and VEGF expression higher in gastric carcinoma than in its normal gastric tissue(P<0.05). Gastric carcinoma tissue with a high expression of TSP-1 showed a low value of MVD and with a high expression of VEGF showed a high of MVD. The correlation among TSP-1,VEGF immunocompetence and microvessel density was highly significant (P<0.05).(4) The over-expression of TSP-1 in gastric cancer was not related to the histological differention, pTNM types, the depth of invasion and tumor size et al, but related to and metastasis of lymph node The over-expression of VEGF in gastric cancer was not related to the histological differention, pTNM types and tumor size et al, but related to the depth of invasion and metastasis of lymph node(5)With high VEGF expression in cancer cells, TSP-1 deposition in stroma appeared to have atendancy to increase.Conclusions: (1) TSP-1 and VEGF is expressing in endochylema of tumor. (2) It is suggested that TSP-1 and VEGF play an important role in neovascularization and invasive growth in gastric carcinoma. Unbalance between the down-expression of TSP-1 and overexpression of VEGF in gastric is one of the key factors for neovascularization in gastric cancer. (3) Combining examination of the expression of VEGF and TSP-1 with MVD should be a better predictor for malignant degree and prognosis in gastric carcinoma, but MVD can exactly respond to biologic action of tumor.