Protective Effect And Anti-oxidize Mechanism Of Astragaloside On Viral Myocarditis In Mice With CVB3

Objective To study protective effect and the anti-oxidize mechanism.of Astragaloside on viral myocarditis in mice with CVB3.Methods Fifty Balb/c mice were randomized into five groups (n=10):normal control group(group A), gived 0.1 mL of EMEM by intraperitoneal injection, were teated with saline 0.1ml with gavage after 30 minities of injection for 1 week; viral myocarditis Control group (group B), inoculated intraperitoneally with 0.1 mL of 1×102 TCID50 CVB3 diluted in Eagle's minimal essential medium (EMEM) solution were given saline 0.1ml with gavage after 30 minities of injection for 1 week; low-dose intervention group (group C), middle-dose intervention group (group D) and high-dose intervention group (group E), inoculated intraperitoneally with 0.1 mL of 1×102 TCID50 CVB3 diluted in Eagle's minimal essential medium (EMEM) were treated with 1%,3%,9% astragaloside(0.07, 0.2 and 0.6g/kg.d,respectively) 0.1mL solution after 30 minities of injection, respectively with gavage for 1 week. Survival rate, score of pathological changes, plasma BNP , the rate of liver and lung's dry weight and wet weight were observed respectively, GSH-PX, CAT, SOD and CUZn -SOD-mRNA of myocardium were detected.Results The survival rate was significantly improved in Balb/c mice treated with high dose astragaloside(group E) than that in viral myocarditis control group (group B)(p<0.01). heart function was better in high-dose intervention group (group E) than in the control group (group B), plasm BNP levels was decreased and the rate of liver's dry weight and wet weight and the ratio of lung's dry weight and wet weight was increased. ,enhance the activity of GSH-PX,CAT ,SOD and CUZnSOD mRNA levels , improved heart function in group E than in control group(group B)(p<0.01). Conclusion The results showed that astragaloside can provide protection against viral myocarditis. This protective effect of astragaloside may be related to the maintenance of the antioxidant status of the heart in improving myocardial antioxidant enzymes activity .