Effect On Survivin Expression In HepG2 Cells By Diallyl Disulfide

Objective: To study the effect on survivin expression in HepG2 cells by diallyldisulfide.Methods: HepG2 cells growth inhibition was measured by MTT assay. The Phasedistribution of cell cycle was analyzed by flow cytometry, survivin mRNA expressionwas detected by reverse transcription polymerase chain reaction (RT-PCR). Theproteins expression of erk1/2, p-erk1/2 and survivin was detected by western-blot.Results: MTT assay showed that DADS significantly inhibited HepG2 cellsproliferation in a concentration-dependent. The low concentration (25μmol/L) DADSdid not induce HepG2 cells proliferation in early phase (6h). The flow cytometry(FCM) analysis showed that there is no apparent G2/M cell population increase at 6hof DADS treatment (25μmol/L) in comparison with the no DADS control. Asignificant increase of G2/M cell population was observed only after DADS treatmentfor 12h or more. Western blot analysis showed that DADS induced survivinexpression. Inhibition of DADS-mediated ERK signaling by PD98059 diminished theexpression of survivin.Conclusion: In the present study, we found that the effect on survivin expression byDADS was related with time. DADS regulated survivin expression by ERK signaling.