| Background and objective Atrial fibrillation, one kind of common arrhythmia in clinical practice,which can lead to disorder of hemodynamics,is a manlay reason of cerebral arterial thrombosis.The development and maintaince of atrial fibrillation is a process which has many fanctors and steps.The research of atrial fibrillation has made much progress recent years,but the mechanism of the development and maintaince still has not been identified completely. Researches reveal that non-steroid anti- inflammatory drug(NSAID) and steroid anti- inflammatory drug(SAID) can cut down incidence rate of atrial fibrillation notablely,which shows that the mechanism have relatively with the inflammatory reaction of cardiac atrium'muscle has been inhibition. However, cluster of differentiation 11b (CD11b) on polymorphoneucler (PMN), interleukin-6(IL-6) and tumor necrosis factor-α(TNF-α) plays a key role in the progress of the inflammatory's happening and development. The research is to approach the relation between every indicatrix with atrial fibrillation's happening and development through investigating the change of the gene expression of cluster of differentiation 11b (CD11b) on polymorphoneucler (PMN), interleukin-6(IL-6) and tumor necrosis factor-α(TNF-α)in right atrial appendage altered in patients with chronic atrial fibrillation.Methods 36 patients with rheumatic heart disease were enrolled. 12 patients had no history of atrial fibrillation and 24 patients had atrial fibrillation,who were all taken cardiac valve replacement by extracorporeal circulation. Atril tissue was obtained from the right atrial appendage during cutting right atrium without extracorporeal circulation,part of the tissue was put in liquid nitrogen immediately, which was transported in refrigerator to deserve.The remaining tissue was put into 10 %formaldehyde, paraffin wax imbedding and cut in slice. The mRNA expression of CD11b,IL-6 and TNF-αwas measured by reversing transcription -polymerase chain reaction(RT-PCR).The protein expression of IL-6 and TNF-αwas detected by immunhistochemical method.The paired t-test was employed to define the change of them,and Pearson correlation analysis was used to define the relationships of the change CD11b,IL-6 and TNF-α.Results 1. Atril tissue and sinus rhythm tissue were detected by immunhistochemical method, and the positive rates of IL-6 were 8.33%(1/12),87.5%(21/24)respectively. Expression integral calculus were 2.10±0.75,0.81±0.81,respectively. Atril groups was significant higher than that in sinus rhythm groups(P<0.05). To combine with expression of intensity, there was significant difference among the 2 groups of cardiac muscle tissue(P<0.001). RT-PCR technique was used to detect tissue, and the limpid straps can be found at the place of 420bp, expression intensity were 1.47±0.47,0.74±0.23, respectively.To compare two groups, the expression of CD11b/GAPDH, significantly increased in patients with chronic atrial fibrillation compared with patients with sinus rhythm. There was significant difference(P<0.001).2. Atril tissue and sinus rhythm tissue were detected by immunhistochemical method, and the positive rates of TNF-αwere 16.67%(2/12),87.5%(21/24)respectively. Expression integral calculus were 2.32±0.81分,1.15±0.70分,respectively. Atril groups was significant higher than that in sinus rhythm groups(P<0.05). To combine with expression intensity, there was significant difference among the 2 groups of cardiac muscle tissue(P<0.001). RT-PCR technique was used to detect tissue, then the limpid straps can be found at the place of 325bp, and expression intensity were 1.72±0.97,0.74±0.28, respectively. Through comparing two groups, the expression of CD11b/ GAPDH, significantly increased in patients with chronic atrial fibrillation compared with patients with sinus rhythm. There was significant difference(P<0.001).3. RT-PCR technique was used to detect the expression of CD11b in cardiac muscle tissue,the limpid straps can be found at the place of 450bp, and expression intensity were 1.49±0.35vs0.75±0.28, respectively.Through comparing two groups, the expression of CD11b/GAPDH significantly increased in patients with chronic atrial fibrillation compared with patients with sinus rhythm. There was significant difference(P<0.001).Conclusion The protein expression of IL-6 and TNF-αsignificantly increased in patients with chronic atrial fibrillation. The mRNA expression of CD11b, IL-6 and TNF-αsignificantly increased in patients with chronic atrial fibrillation Inflammation may be one of mechanisms for the development and maintainance of atrial fibrillation, which is a new way to reduce expression and concentration of CD11b,IL-6 and TNF-α.
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