The Effects Of Smecta On Intestinal Microflora And Immune Function In Experimental Mice

The experiment was conducted with objectives of dividing one hundred andfifty KUNMING origin white mice into five groups at random in the experiment: normalcontrast group, model group, natural cure group (negative contrast group), Smectagroup(experiment group) and Medilac-Vita group (masculine contrast group), with 30mice in each group. Expect that the mice in normal contrast group were given the equalSodium Chloride, the other were given Lincomycin hydrochloride, Cefazolin sodium andampicillin sodium made by the PH7.4 Sodium Chloride at the same time(the threeantibiotics are 100mg/ml respectively), 0.2ml per mouse, twice per day, interval 8 hours, andfor 3 days. After the mice occured intestinal dysbacteriosis, except that the mice in naturalcure group were given the equal Sodium Chloride every day, the other in Smecta groupand Medilac-Vita group were given 6%Smecta and Medilac-Vita(0.2 a hundred millionviable organism per ml), 0.2 ml per mouse, twice per day, interval 8 hours, and for 10 days. Onthe eleventh day, the feces of the mice in each group were collected for quantity detectionabout BS, LBS, EMB and EC in intestines; Detect the content of IgA,IgG,IgM in serum,T lymphodyte transformation efficiency and mononuclear macrophage phagocytosisefficiency by collecting blood from heart after execute the experimental mice; Pick thethymus and spleen to detect the indexes of immune organs; Observe the changes ofpathologic damage about the colons of the mice in each group at the same time. It was tostudy the effects of Smecta on intestinal microflora and immune function in animals, andalso protection about the intestines. It would provide a new method to cure intestinal dysbacteriosis by the antibiotic; At the same time, it also had the theory direction meaning.According to the practice conditions for veterinary clinic, the experiment simulated theclinical cases different from the previous methods to make pathology model, which wascured after intestinal dysbacteriosis by 3 different antibiotics. So the experiment had moredirection meaning for clinic.The results showed that after being given generous antibiotics, the mice had intestinaldysbacteriosis, and the quantity of BS,LBS,EMB and EC significantly decreased (P＜0.01); the indexes of immune organs, body fluid immune, cell immune and unspecificityimmune also significantly decreased after intestinal dysbacteriosis (P＜0.01); The quantityof intestinal bacteria and immune function of the mice increased a little after natural curefor 10 days, but they were lower than that in normal contrast group (P＜0.01 or P＜0.05);In Smecta group and Medilac-Vita group, the quantity of BS,LBS,EMB and EC inintestines of the mice significantly increased, and it was no significant differencecomparing with that in normal contrast group(P＜0.01); The content of IgA, IgG, IgM inserum, T lymphodyte transformation efficiency and mononuclear macrophagephagocytosis efficiency of the mice in two cure groups were higher than that in normalcontrast group significantly (P＜0.01 or P＜0.05); The indexes of immune organs of themice was no significant difference comparing with that in normal contrast group(P＞0.05); And the pathology damage about the colons of the mice in two cure groupsrecovered significantly, however, that in natural group wasn't significant.It is concluded that after intestinal dysbacteriosis, Smecta can regulate the quantity ofintestinal microflora well in the body, and help recover balance of the microecology inintestines; The immune function decreases by intestinal dysbacteriosis, but Smecta canstrengthen that in the body, and recovers pathology damage about the colons of the animals.So, Smecta could be one of new drugs after microflora praeparatum for curing intestinalbacterium by the antibiotic.