Effects Of "QiLing" Medicament Polysaccharide On Intestinal Mucosal Immunity And Immune Regulation In Immunosuppressed Mice

150 mice were randomly divided into 5 groups, including control group, cyclophosphamide (CY) model group and 3 different doses of"QiLing"medicament polysaccharide groups (low dose+CY group, middle dose+CY group and high dose+CY group). Each group contained 30 mice. Control group and CY model group were gavaged with distilled water, and"QiLing"medicament polysaccharide groups were gavaged with different doses of"QiLing"medicament polysaccharide (200 mg/kg·BW,400 mg/kg·BW,600mg/kg·BW, respectively) for 19 days. Then control group was injected with saline solution, and the other groups were injected with cyclophosphamide(CY) with a dose of 100mg/kg·BW. By detecting intestinal microflora, intestinal mucus secretory immunoglobulin A (SIgA) and by observing intestinal mucosal morphology and variations of cell number in associated tissue, and detecting immune indicators (serum immunoglobulin, complement, immune organ index and serum lysozyme, etc.) and serum antioxidant levels, intestinal mucosal immunity and the immune regulations of"QiLing"medicament polysaccharide on immunosuppressed mice were mainly studied. The results were as follows:(1) Flora changes of intestinal contents showed that the number of Bifidobacterium, Lactobacillus were significantly increased, and the number of E.coli, enterococci were significantly decreased(P < 0.05 or P < 0.01) in"QiLing"polysaccharide groups compared to the CY model group. This means that"QiLing"polysaccharide could regulate intestinal flora disturbance caused by CY.(2) The content of SIgA in intestinal mucus in CY model group were significantly decreased,and the content of SIgA in intestinal mucus in polysaccharide groups were significantly increased (P<0.05 or P<0.01), suggesting that to some extent,"QiLing"polysaccharide could regulate and antagonize CY-induced damage of intestinal mucosal immunity. (3) Histological observations showed that length of intestinal villus, villus length and crypt depth ratios (V/C), the number of intestinal intraepithelial lymphocytes and mucous cells were all reduced in CY model group. These indicators of polysaccharide groups were kept normal or were obviously incresed comparing to CY model group. The results indicated that the polysaccharide could prevent the damage of cyclophos- phamide on intestinal mucosal immune, and strengthen the intestinal mucosal immune function.(4) The detection of serum immune indicators showed that, the contents of immunoglobulin, complement, protein lysozyme in serum and immune organ index of CY model group were significantly lower than that of control group(P<0.01). The contents of immunoglobulin, complement, protein lysozyme in serum and immune organ index of polysaccharide groups were significantly increased (P<0.05 or P<0.01) comparing to the CY model group. That indicated that"QiLing"polysaccharide could imrpove immune function and antagonize antagonize immunosuppressive effects of cyclophosphamide in mice.(5) The activities of SOD and MDA in serum showed that significantly differences were observed in serum SOD activity and MDA content between CY model groups and control group(P<0.01).And there were significantly differences in serum SOD activity and MDA content between CY model group and polysaccharide groups(P<0.01). It suggested that"QiLing"polysaccharide could improve the ability of antioxidation of the immune suppressed mice.