Polymorphic Microsatellite Marker Of The Penicillium Marneffei In Guangxi

Objectives To investigate the gene polymorphism of Penicillium marneffei (PM)in Guangxi,and investigate the difference of the gene polymorphism in Guangxi PM between the isolates obtained from Rhizomys pruinosus and those from penicilliosis marneffei(PSM)patients in Guangxi.Methods Total DNA was extracted with benzyl chloride.Polymorphic microsatellite marker(PMM)analysis was used to study the gene polymorphism of 40 isolates penicillium marneffei obtained from Guangxi Rhizomys pruinosus and 31 isolates PM obtained from PSM patients in Guangxi.Results①The natural positive carrier rate of the 40 was 100%.②Polymorphic microsatellite marker analysis detect a total of 53 different allelic types for 71 isolates of PM with a high discriminatory power(D＝0.990)。MicrosatellitesⅠ,ⅡandⅢdetected 21,10,and 9 alleles,respectively.③The genetic identity of PM obtained from Rhizomys pruinosus in various districts of Guangxi is between 0.1217and 0.4464.④The genetic identity of Guangxi PM between the Rhizomys pruinosus and PSM patients is 0.8689.⑤The genetic identity is between 0.0604 and 0.8418 in the PM from the different districts of Guangxi Rhizomys pruinosus and PSM patients.Conclusions There is the gene polymorphism in the PM from Guangxi;There is the geographical gene polymorphic in the PM from the Rhizomys pruinosus in different districts of Guangxi;The pathogens cause of the hunman PSM probably closely related to the Guangxi Rhizomys pruinosus PM.