| Objectives To investigate the differential expression of the GasC gene in Guangxi Penicillium marneffei (PM) between the isolates obtained from Rhizomys pruinosus and those from Penicilliosis marneffei(PSM) patientsMethods Selected randomly 30 isolates separately obtained from Rhizomys pruinosus and those from PSM patients cultured in SDA liquid medium at 25℃after 72h. Total RNA was extracted with Trizol. Detect the expression of GasC gene in each isolates through the fluorescence quantitive PCR technic. Analysis the difference of the GasC expressed in the two group by SPSS.Results①The natural positive carrier rate of the 15 was 100 %, 30 isolates can all produced the red pigment at 25°C after 72h cultured in SDA liquid medium,and they all produced the red pigment with different level,but no difference in statistics between the two group (p>0.05) .②we can see the goal gene through the gelelectrophoresis③Detect the expression of GasC gene in each isolates through the Real Time PCR and there is not difference in statistics between the two group (p>0.05) .④The expression of GasC gene is posertivly connect with the ability for producing the pigment of PM, and the deeper the pigment shows , the higher the level of GasC gene expressed.Conclusions There is no difference in producing red pigment between the isolates obtained from Rhizomys pruinosus and those from Penicilliosis marneffei(PSM) patients; The expression of GasC gene is not difference in statistics between the two group which preliminary revealed the similarity in growth vitality and pathogenicity;The expression of GasC gene is connect with the ability for producing the pigment of PM, and the deeper the pigment shows , the higher the level of GasC gene expressed. It must be proved by the further experiment on animals whether the PM with deeper pigment and the high expression of GasC gene presents stronger pathogenicity.
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