The Expression Of Osteopontin And UPA And Its Relationship With Prognosis In Non-small Cell Lung Cancer

Objective: In this study, immunohistochemistry was used to investigate expression situation of OPN and uPA proteins in NSCLC specimen, analyzed its relationship with clinical pathological factors and the survival time after patient suffered surgical resection. The purpose was to study their roles in invasion and metastasis of NSCLC and provide new theoretical foundation for forecasting the early diagnosis, prevention and clinical treatment, judging prognosis of NSCLC.Methods: Samples of 45 NSCLC and 15 normal lung tissues were detected by Supervision? two-step method for OPN and uPA proteins expression. SPSS (13.0) statistical software was applied for data analysis. A P-value less than 0.05 were considered significant. The correlation between the expression of OPN and uPA and the major clinical pathological characteristics of NSCLC were analyzed by Chi-square test and spearman analysis of correlation. Postoperative survival period analysis were computed by the Kaplan-Meier method and compared by the Log-rank test. The prognostic relevance of OPN and uPA and conventional prognostic factors were analyzed by Cox's regression model.Results: 1. The positive expression rate of OPN and uPA in NSCLC specimens was 71.1% and 62.2%, respectively. Positive rate of OPN and uPA expression in normal lung tissue was 6.7% and 13.3%, respectively. The different positive rates between NSCLC and normal lung tissue had statistical significance (P<0.05). 2. The expression of OPN and uPA proteins was not correlated with gender, age, pathological types, size of tumor and TNM stages (P>0.05). 3. Positive expression rate of OPN was 54.5% in the non-lymph node metastasis groups and 87.0% in the lymph node metastasis groups, the difference had statistical significance (P<0.05); Positive expression rate of uPA in the two groups was 45.5% and 78.3%, respectively, the difference had statistical significance (P<0.05). 4. Positive expression rate of OPN in high, center, and low histological grades was 60.0%,73.3% and 80.0%, respectively, the difference had statistical significance(P<0.05). Positive expression rate of uPA in high, center, and low histological grades was 40.0%,60.0% and 86.7%, respectively, the difference had statistical significance (P<0.05). 5. There were significantly positive correlationship between OPN expression and uPA expression in NSCLC (r_s=0.321, P<0.05). 6. The histological grades, lymph node metastasis status, TNM Stages, OPN expression, and uPA expression were correlated with prognosis. Patients with lower histological grades, lymph node metastasis, and later TNM stages associated OPN and uPA expression singly or their combinated expressions were significantly suggested unfavourable prognosis.7. Multivariate analysis of prognostic factors in NSCLC patients was carried out by Cox's regression model, found that lymph node metastasis status, TNM Stages, OPN expression, and uPA expression were risk factors which affect survival time of patients (P<0.05). Conclusion: 1. The positive rate of OPN and uPA expression in was higher in NSCLC tissue than that in normal lung tissue. High positive rate of OPN and uPA expression in NSCLC provide a theoretical basis for diagnosis. 2. OPN and uPA expression was not correlated with gender, age, pathological types, size of tumor and TNM Stages, and was related to histological grades and lymph node metastasis status of NSCLC patients. The results suggested that OPN and uPA expressive were prone to local invasion and lymph node metastasis. 3. Median survival period of patients with OPN or uPA positive was significantly shorter than that with OPN or uPA negative. The result showed that OPN and uPA expression were associated with short survival and poor prognosis. 4. There was significantly positive correlationship between OPN and uPA expression in NSCLC. Their combined expression had the risk of decreasing survival time of patients with NSCLC. 5. The lymph node metastasis status, TNM Stages, OPN expression, and uPA expression were correlated with prognosis, and they were independent risk prognostic factors. Detecting OPN expression together with uPA expression provided a reference to evaluate malignant potential and prognosis of NSCLC.