Expression Of Heparanase, Syndecan-1, VEGF Protein And Their Relationship With The Invasion And Metastasis Of Gastric Carcinoma

Objective To investigate the expression of heparanase, syndecan-1, VEGF protein and their correlation in gastric carcinoma, their relationship with the invasion and metastasis of gastric carcinoma.Methods Elivision immunohistochemistry technique was used to detect the expression of heparanase,syndecan-l,VEGF in sixty specimens of gastric carcinoma, sixty specimens of gastric adjacent tissue to tumor and thirty specimens of normal gastric tissue. SPSS13.0 statistical package was used to analyze the relationship between the expression of heparanase, syndecan-1, VEGF protein and the clinicopathological features in gastric carcinoma.Results (1)The positive rate of the expression of syndecan-1 in gastric carcinoma, gastric adjacent tissue to tumor and normal gastric tissue were 68.33%, 35.00%, 0%, respectively(P<0.01). It was significantly related to TNM stages, lymph node metastasis, infiltration depth, distant metastasis(P<0.01 ),but it was not related to age, gender, tumor size, tumor location, differentiation, histological type and Borrmann type (P>0.05) .(2)The positive rate of the expression of syndecan-1 in gastric carcinoma, gastric adjacent tissue to tumor and normal gastric tissue were 26.67%, 53.33%, 76.67%, respectively (P<0.05). The positive rate of syndecan-1 expression in normal gastric tissue was significantly higher than that in gastric adjacent tissue to tumor and gastric carcinoma. It was significantly related to TNM stages, lymph node metastasis, distant metastasis (P<0.05) ; but it was not related to age, gender, tumor size, tumor location, infiltration depth , differentiation, histological type,Borrmann type (P>0.05) .(3) The positive rate of the expression of VEGF in gastric carcinoma, gastric adjacent tissue to tumor and gastric normal tissue were 70.00%, 48.33%, 6.67%, respectively (P<0.05) . It was significantly related to TNM stages, lymph node metastasis, infiltration depth, distant metastasis (P<0.05) ,but it was not related to age, gender, tumor size, tumor location, infiltration depth, differentiation, histological type, Borrmann type (P>0.05) (4) There was a negative correlation between the positive expression of heparanase and syndecan-1 in sixty gastric carcinoma specimens (r=-0.289, P<0.05) , and there was a significant positive correlation between the expression of heparanase and VEGF (r=0.401, P<0.01) , but there was no correlation between the expression of syndecan-1 and VEGF in gastric carcinoma(r=-0.152, P>0.05).Conclusion (1)The expression of heparanase and VEGF in gastric carcinoma tissues was much higher than that in gastric adjacent tissue to tumor and in normal gastric tissue. The expression of syndecan-1 was much higher in normal gastric tissue than that in gastric adjacent tissue to tumor and in gastric carcinoma. It suggests that they may play an important role in gastric Carcinogenesis and progression. (2)The high expression of heparanase and VEGF in gastric carcinoma tissues closely related to TNM stages, lymph node metastasis, infiltration depth, distant metastasis, the low expression of syndecan-1 in gastric carcinoma tissues closely related to TNM stages, lymph node metastasis, distant metastasis, which suggest that they may be the powerful predictors in evaluating tumor malignant biological behaviour. (3) There is a negative correlation between the positive expression of heparanase and syndecan-1 in sixty gastric carcinoma specimens, and a significant positive correlation between the expression of heparanase and VEGF, but no correlation between the expression of syndecan-1 and VEGF in gastric carcinoma. Combinative detection of heparanase, VEGF and syndecan-1 expression in gastric carcinoma may be value to tumor prognosis and malignancy extent, and to provide some experimental and clinical evidences for the treatment of gastric carcinoma.