Induction Of Dedifferentiation In Macroglial Cells And Its Mechanism In Injuried Optic Nerve Of Rats

To explore the effects of bFGF, CNTF and hair follicle neural stem cells on dedifferentiation of macroglial and the feasibility of induction of the regeneration following optic nerve injuries, bFGF, CNTF and hair follicle neural stem cells were injected to the damaged optic nerve of rats respectively. Focusing on changes of morphology and expression of proteins and genes, HE staining, immunohistochemistry, gene microarry and Real-time quantitative PCR has been processed. The results show that the number of cells in optic nerve administrated with bFGF,CNTF and hair follicle neural stem cells increased.Some genes related to apoptosis or proliferation changed to protect the glias and improve their proliferation. Expression of the markers of neural stem cells increased;meanwhile some stem cell-related genes and these related to development and differentiation were up-regulated.ALL of these indicated the trend of dedifferentiation of macroglias in injuried optic nerve.Besides, expression of genes related to growth and growth factors had increased which can improve the microenvironment that inhibited the regeneration of optic nerve.But they have different influence on macroglias,through regulating expression of different genes.The results give a new eye of repairing optic nerve injuries and better understand of the mechanism of dedifferentiation.