Effects Of Fuyuan Capsule On Synovium In Experimental Osteoarthritis Of Rabbit

OBJECTIVE:To observe the effects of fuyuan capsule on change of morphology of synovium,expression of IL-1β,MMP-13 and iNOS in synovium in experimental osteoarthritis of rabbit,and investigate the mechanism about the reparation and treatment of fuyuan capsule.METHODS: Fifty-four New Zealand rabbits were divided into five groups randomly:normol group,model contrast group,low dose group of fuyuan capsule,middle dose group of fuyuan capsule and high dose group of fuyuan capsule. OA model was induced in rabbits by improved Hulth'S method.Three Chinese herb groups were treated with fuyuan capsule(0.727g.kg-1.d-1,1 .453 g.kg-1.d-1,4 .795 g.kg-1.d-1),ig, continuously for 4 weeks,8 weeks and12 weeks. model contrast group was treated with 0.9%NS ,ig,at the same time. Synovium sections were analyzed by immunohistochemistry for the IL-1β,MMP-13 and iNOS,observation and measurement of the change of morphology of synovium by light microscopic and transmission electron microscopic . After the experiment,the differences of these indexes between Three Chinese herb groups and model contrast group were compared. RESULTS: There are found significant differences about measurement of morphology of synovium between Three Chinese herb groups and model contrast group after the experiment(P < 0.05),especially high and low dosage of fuyuan capsule group(P<0.01).It is the same as the comparison of the expression of IL-1β,MMP-13 and iNOS.CONCLUSIONS: Fuyuan capsule can improve the pathological change of morphology of synovium,and decrease the expression of IL-1β,MMP-13 and iNOS in synovium in experimental osteoarthritis of rabbit. There was therapeutical effect on synovium in experimental osteoarthritis of rabbit.