The Research For Protection Of Fuyuan Capsule On Osteoarthritis Chondrocytes Via JNK Signaling Pathway

ObjectiveTo investigate the protective effect and the mechanism of Fuyuan capsule on Osteoarthritis'apoptosis of cartilage cells and cartilage matrix degradation.MethodsHuman chondrosarcoma cells were cultured and divided into 5 groups randomly:1) normal group,2) model group,3) inhibitor group,4) Fuyuan capsule group,5) Fuyuan capsule+inhibitor group. The percentage of cell cycle and apoptosis index was detected by Flow cytometry. The activity of cell proliferation was detected by CCK-8. The expression of MMP-3,-13 mRNA was determined by Q-PCR. The expression of cell signaling factor of p-JNK and Bax was determined by Western-blot.ResultsThe model group compared with normal group, the percentage of Go/G1 phase increased, the percentage of S phase and G2/M phase decreased, the PI decreased; the apoptosis rate was significantly increased; the activity of cell proliferation decreased; the expression of MMP-3,-13 mRNA andp-JNK, Bax protein were significantly increased (P<0.01). The intervention group compared with the model group, the PI increased; the apoptosis rate increased significantly; the activity of cell proliferation increased; the expression of MMP-3,-13 mRNA and p-JNK, Bax protein were decreased (P<0.01 or P<0.05); the Fuyuan capsule+inhibitor group was most significantly compared with the model group(P<0.01).ConclusionFuyuan capsule can inhibit the apoptosis and promote the proliferation in OA cartilage cells; Fuyuan capsule can inhibit the degradation of extracellular matrix; Fuyuan capsule is stronger than the specific inhibitor of JNK single in the prevention and treatment of osteoarthritis; Its mechanism may be related to the inhibition of the JNK signaling pathway.