Expression Of HOXa-10 Gene In The Endometrium Of Early Pregnancy Mouse And Its Function

Background:The blastocyst implantation is the process of fertilized egg grows to the blastocyst and hatches from the transparent belt stage, then it accomplish adherency and invasion in endometrium. It including the embryo trophoderm and the endometrium epidermis recognition,cell adherency and the germinal vesicle to the endometrium invasion and so on, that determine whether pregnancy will develop successfully or not. In'window of implantation', ovarian hormone stimulate endometrium epidermis and matrix multiplication and differentiation: The cavity epidermis turns the condition which is suitable for to stick extremely, but intersititial cell preparation to decidua cell transformation. The pouch embryo adherency urges around the embryo in the cavity epidermis to have the decidua to respond that, the endometrium is at'acceptivity'the condition, the blastocyst this time very is easy to complete implantation. But the acceptive endometrium produces the molecular mechanism is not clear. In recent years studied the discovery, HOXA-10 gene all is having certain influence to the acceptive endometrium formation and blastocyst implantation. Homeobox gene (HOX) is McGinnis[1] and Scott[2] which discovered in fruit fly in vivo the related with the fruit fly body festival changes a kind gene, belongs to the multi-gene family nuclear transcription regulatory gene, through activates or the suppression goal gene with the DNA union, its important function is decides the cell the directional differentiation and the multiplication. The HOX gene includes an approximately 183 nucleotide composition homologous frame sequence, codes 61 amino acid constitutions the homologous territory structure, through the screw - corner - screw structure took DNA the union territory, (TTAT or TAAT) distinguishes in the downstream target gene DNA sequence, thus activates or suppression downstream gene copying[3]. HOXA-10 gene gradually received in recent years in reproduction process function focuses attention on, is considered one of for implantation regulatory genes. Therefore, Hoxa-10 gene in the pregnant mouse endometrium expression rule is regard having the necessity to research the influence of the blastocyst implantation.Objective:Studies the rule of Hoxa-10 gene and protein in early pregnant mouse endometrium expression, and discusses it function in the process of blastocyst implantation. Methods:1.Collecte the no pregnant D0 and in early pregnant D1,D2,D3,D4,D5 and D7 mice endometrium, altogether 7 groups, each group of 10 mouse. Real-time fluorescence quantitative PCR (FQ-PCR) and immunohistochemical analysis separately examine various issue of mouse endometrium HOXa-10 gene and protein expression;2.Transfection with DNA/ liposome compound distinction extension to be possible to expression HOXa-10 gene continually the material particle and to becomes in D2 pregnant mouse uterus angle in view of HOXa-10 antisense oligonuleotides/ liposome in pregnancy D2 mouse horn of uterus respectively, and detected the variety of quantity in comparison with normal pregnancy D2 mouse blastocyst nidation. We take normally pregnant D2 mouse as the comparison, contrasts three group of mouse's blastocyst nidation numbers.Result:FQ-PCR obtains along the mouse pregnancy days number with increase, the endometrium HOXa-10 gene expression quantity also gradually advances, is being pregnant D4 to achieve the peak value, is pregnant D5 start to droping, weather in pregnant D7 its expression closing the no pregnant condition, D0,D1 and D7 ampho-compare P>0.05, other group ampho-compare P<0.05. Immunohistochemical analysis showed the same results as FQ-PCR, but it have different organization distributed characteristic in the different time.Transfection with HOXa-10 DNA / liposome compound in D2 pregnant mouse uterus angle, the rate of implantation obvious increased; Transfection with HOXa-10 antisense oligonuleotides / liposome compound in D2 pregnant mouse uterus angle, the rate of implantation is reducing obviously. The result analysis made use of the statistical analysis software SPSS12.0. Research has revealed each group of differences significance. Conclusion:HOXa-10 gene from is pregnant D1 in mouse endometrium expression in pregnant to have a drop process D5 gradually, it with blastocyst implantation when the endometrium decidualization process is completely consistent. It possible is HOXa-10 promoted the intersititial cell to the decidua cell transformation, Then is advantageous in enhancing implantation'the window time'endometrium receptivity.It creating the condition for the blastocyst implantation. Transfection with DNA/ liposome compound distinction extension to be possible to express HOXa-10 gene continually the material particle and after becomes in D2 pregnant mouse uterus angle in view of HOXa-10 gene antisense oligonuleotides, Separately strengthened and has suppressed HOXa-10 gene expression, two groups were pregnant mouse's the rate of implantation also obviously different. It is explained HOXa-10 gene was having the vital role in the blastocyst implantation process.