Study Anticancer Activity Of Quercetin And Expression Of MDR₁ And P-GP In HepG₂ Cells

Object: To observe the effect of quercetin on growth inhibiting of HepG2 cells and expression of MDR1 AND P-gp in vitro, study the mechanism and investigate whether it can improve the chemical therapeutic effect of liver cancer.Method: HepG2 cells were treated with different concentration of quercetin for different time. The inhibitory effect of quercetion on cell growth was observed by MTT-assay. TUNEL detected the effect on apoptosis of HepG2 cells exposed to quercetin for 48h. The expression of MDR1 AND P-gp were detected by RT-PCR and immunocytochemistry. Result: (1) HepG2 cells growth were inhibited by quercetin. The inhibitory degree depended on exerted concentration and time(P<0.01). With the dose increased and time extended, the inhibitory effect was enhanced. (2) Quercetin could induced the apoptosis of HepG2 cells. Comparison between high concentration group(100uM,200uM)and low concentration group ( 50uM ) and control group have significance(P<0.01).(3) The expressing of MDR1mRNA of HepG2 cells could be inhibited by quercetin. The inhibitory degree depended on the exerted concentration(P<0.01). But with time go on, the inhibitory effect disappeared. (4) The expressing of P-gp of HepG2 cells could be inhibited by quercetin. The inhibitory degree depended on the exerted concentration(P<0.01). After HepG2 cells was exposed to quercetin for 72h, the difference from group of 50uM,100uM and 200uM have no significant(P>0.05), but compared with the control group, it have significan(tP<0.01).Conclusion: 1.It is one of mechanism of action that through inducing apoptosis, quercetin can inhibit the HepG2 cells proliferation. 2. The expressing of MDR1mRNA of HepG2 cells can be inhibited by quercetin. But with time go on, the inhibitory effect disappears. 3. Through inhibiting the expression of MDR1mRNA of HepG2 cells, quercetin can inhibit the expression of P-gp of HepG2 cells. 4. Quercetin could be a selection to improve the chemical therapeutic effect of liver cancer. 5. The mechanism of action of quercetin is complicated, it need more experiments to be comprehended.