Extraction Of Anti-bacteria Substance From The Hybrid Edible Gourd And Study On Anti-bacteria Effect Of The Extraction

The results of anti-bacteria experiments with the hybrid edible gourd and the balsam pear or the edible gourd indicate that the hybrid edible gourd has the very strong anti-bacteria effect。Extracting the anti-bacteria components from the hybrid edible gourd with the different solvent,components ectracted with water have the best anti-bacteria effect。The best condition of anti-bacteria components extraction is as follows. After juice of the hybrid edible gourd extracted, remains of the hybrid edible gourd and the water are mixed according to 1:14 proportion . The mixture is kept at 50~60℃for 80mins, and that extraction is repeted 2 times, the anti-bacteria ingredient in the hybrid edible gourd is basically extracted. Anti-bacteria ingredient can be purified effectly When the extraction of the hybrid edible gourd is treated with ethyl alcohol at low temperature . When Ph is 4.0~9.0, this purification has the bacteriostatic effect. HD-0 and HD-6 which have bacteriostatic effect are separated from the purification with the pocket absorption resin.HD-0 is best processed with the activated charcoal for 60mins and HD-6 is done in the same way for 40mins. HD-0 Processed with the activated charcoal is treated with the silica gel column under the following conditions. The best solution of the silica gel column is the mixture of chloroform and ethyl acetate according to 1:9 proportion and the best eluent is gradient eluent compounded by chloroform,ethyl acetate and ethyl alcohol according to different proportions. HD-6 Processed with the activated charcoal is treated with the silica gel column under the following conditions.The best solution of the silica gel column is the mixture of chloroform and ethyl acetate according to 1:1 proportion and the best eluent is gradient eluent compounded by chloroform and ethyl acetate according to different proportions. HD-0 or HD-6 is separately analysed through the method of the silica gel column chromatographic analysis under the optimum conditions. Three kinds of anti-bacteria component (HG0-1, HG0-2 and HG0-3) are obtained from HD-0 and two kinds of anti-bacteria component (HG6-2,HG6-3) are obtained from HD-6. The silicon off-set thin layer chromatographic analysis indicates that five substances(HG0-1, HG0-2, HG0-3, HG6-2 and HG6-3) are bacteriostatics containing sole components. That five substances are initially judged as the flavanone accrding to the color reaction experiment. HG0-1, HG0-2, HG0-3, HG6-2 and HG6-3 obtained by the silica gel column separation analytic way have the bacteriostasis effect on the bacterium (backwoods coli, golden yellow staphylococcus, subtilis bud spore bacillus, enteritis salmonella bacillus, typhus salmonella bacillus, dysentery will He fungus, the Liszt bacillus) and the black tune mildew. The lowest bacteriostasis density of the above-mentioned bacteria is 6.0~ 12.0mg/mL.