Studies On The Anti-viral Effects Of Five Formulas From Yao And Zhuang Minority

ObjectiveAnti-viral, anti-bacterial, anti-inflammatory, analgesic and antipyretic effects ofextracts of five formula from Yao and Zhuang minority were investigated.Methods(1)Five formulas from Yao and Zhuang minority were decocted. Ethanol was added tothe decoctions and the dissoluble parts were collected and concentrated.(2)The anti-influenza-viral activities of the extracts were tested by cytopathic effectassay (CPE).(3) The minimal inhibitory concentraction (MIC) of the extracts against some common bacteria and theirs clinical isolates of bacteria were tested by stardard agar dilution method.(4) Mouse ear swelling induced by xylene was used to observe the anti-inflammatory effect of the extracts.(5)Febrile model induced by injecting yeast subcutaneously was used to observeantipyretic effect of the extracts.h(6)Mouse body twist induced by acetic acid was chosen to observe the analgesic effectof the extracts.Results(1)The extracts were obtained from five formulas of Yao and Zhuang Miniotory(aqueous extracts).(2)The result of CPE shows Formula1,2,5 could inhibit the cytopathic effect induced byinfluenza virus, IC₅₀ were 10.69mg /ml, 3.74mg /ml and 8.42mg /ml, TI were morethan1.73,2.32 and 2.32 respectively.(3)The experiment of antibacterial activity in vitro indicates Formula 1,2,4,5 havecertain antibacterial activity against Steptococcus pneumoniae, MIC were 125mg /ml,125～250 mg/ml, 62.5～250mg/ml and 250～500 mg/ml respectively.(4)The five formulas could lessen mouse ear swelling induced by xylene,but the effectof high-dose group of formula 1,2 were more significant than others.(5)Formula 1,2,3,4,5 could inhibit the increases of animal temperature induced by pyrogen.(6)Formula 1,3,5 could inhibit writhing response induced by acetic acid.ConclusionFormula1,2,5 have certain anti-viral, anti-bacterial, anti-inflammatory, analgesic and antipyretic effects. They are of value for further study and development.