Acute Exacerbation Of COPD And Remission In Patients With Serum IL-4, IL-8, IFN-γ Concentration And Clinical Significance Of Changes

PrefaceChronic obstructive pulmonary disease (COPD), respiratory diseases, with a not Entirely lung diseasesNot exactly the pathogenesis of COPD, the mechanisms involved in cellular and molecular biology, protease - anti-system, the role of oxidants, infection. COPD airway inflammation by immune cells (inflammatory cells) produce a variety of cytokines mediated interaction and a chronic inflammation. IL-8 is the first study of cytokines in the pathogenesis of COPD play an important role. IL-8 is an important PMN chemokines may induce morphological changes in the intracellular Ca2 of the increased promotion of degranulation and the release of lysosomal enzymes and bioactive lipid formation and respiratory burst, forming super oxide, thus promoting inflammation. Major TH1 cells secreting IL-2, IFN-γ, it is primarily mediated immune response, anti-infection, organ transplant rejection and autoimmune diseases induced in the process plays an important role, mainly TH2 cells secreting IL-4, mainly mediated by humoral immune responses, Auxiliary antibody, in a leading role in the allergic reaction to the B-cell antigen-specific IgG1 and IgE antibody secretion. Th1 and Th2 cells by the immune response induced by regulation or co-produced cross-regulatory role of IFN-γcan inhibit the proliferation of Th2 cells, IL-4 and IFN-γcan be reduced responsiveness.To explore the pathogenesis of COPD, in this experiment, we tested elderly patients with chronic obstructive pulmonary disease (COPD) and acute exacerbation remission in patients with IL-8, gamma-interferon and interleukin-4 (IL-4) in serum values, to explore COPD patients with acute exacerbation and remission of the immune mechanism for acute exacerbation of COPD reasonable treatment and provide a theoretical basis. Object and Method1.ObjectChoose from COPD group from August 2005 to March 2006 in our hospital hospitalized patients with acute exacerbation of COPD and the control group were 20 cases of physical examination.2.equipment, reagents(1) Finland Labsysbens wholly-type enzyme.(2) IL-8 by the Biotechnology Limited Haizhuokang Kit(3) IFN-γ, IL-4 Beijing Crystal US biotechnology companies kit, operation manuals for the kit.(4) Lung Function Made In Japan Repi-Spirolab3.methods(1) samples collected from hospital after fasting 6 milliliters of blood, injection tube, centrifugal 2500rpm. 15℃to 20℃, 10～20 min, the serum Add to draw ependoff tube, -70℃to prepare for reunification cryopreservation detection. COPD remission further 6 ml of fasting blood taken immediately with the centrifugal method serum, or in the refrigerator preserve -70℃to be ofIL-8, IFN-γ, IL-4.(2) Experimental Methods double-antibody sandwich ELISA.Results1.IL-8 (pg / ml): COPD acute phase (245.19±82.23), remission (103.66±33.12) than the control group (32.95±10.02) was significantly increased and urgent COPD and remission period of IL-8 also statistically signifance difference (p <0.01).2.IL-4 (pg / ml): acute exacerbation of COPD (80.95±13.67) than remission (25.56±8.6) and the control group (51.46±10.20) was significantly higher (P < 0.01).3.IFN -γ(pg / ml): acute exacerbation of COPD (22.36±4.46) than in remission period (16.76±4.04) and the control group (17.62±4.78), but no significant significance.4.Acute exacerbation of COPD IL-8(245.19±82.23) were negatively correlated with FEV1 ( 1263±461 ) (r=-0.76,P <0.01). DiscussionCOPD is the main pathologic basis of airway inflammation in chronic non-specific, the main characteristics is within the lumen neutrophil aggregation main wall of the main T lymphocyte infiltration, IL-8 as a neutrophils and T lymphocytes chemokines, in inflammatory reactions play an important regulator role. In the lungs, mainly derived from alveolar macrophages, airway epithelial cells, lymphocytes and neutrophil aggregation, IL-8 may tolerance serum and effusions Endopeptidase degradation, there a long time, which can be accumulated in local, in response to a sustained effect, enlarged inflammatory response . This study shows that acute exacerbation of COPD plasma IL-8 levels higher, indicating acute exacerbation of COPD in the presence of IL-8 produced excessive, excessive expression. The results showed that patients with acute exacerbation of COPD treatment serum levels of IL-8 than after treatment (p <0.01), by the anti-inflammatory treatment, airway inflammation under control, serum IL-8 levels significantly lower than before treatment, suggesting that IL-8 gas directly involved in COPD Road inflammation process, and the results showed that after treatment serum levels of IL-8 than the normal control group and the significant differences, which may ease with COPD and there is still 25% of the bacterial infection. FEV1 decline before treatment, in remission period increased FEV1, and IL-8 was negatively correlated with the IL-8 and the restricted airflow.TH2 cells stimulate the humoral immunity, the secretion of IL-4 can promote B-cell proliferation and induce the formation of antibodies, particularly IgE production, the main TH1 cells secreted IFN-γ.IL-2 and TNF-a, TH1 to remove the source of infection for the body, including hidden in the bacterial source of infection, the group observed that the level of IL-4 was significantly higher than that of IFN-γlevels before treatment is higher than that trend, the ratio compared with the control group IFN-/γIL-4 reduce body for the performance of state TH2 advantage of the trend, relief periods when IFN-r level lower than the control group, the level of IL-4 was significantly lower than the control group, after treatment 1FN-γ/IL-4 ratio compared with the control group, IFN-γTH1 activity and can improve the level, so the body for the performance advantages of TH1 state. Before treatment performance advantages for the state TH2 after treatment for the performance advantages of TH1 state, the treatment of acute exacerbation of COPD around the state TH1/TH2 advantages exist significant differences, and IL-4, IFN-γdirectly related changes.Conclusion1 acute exacerbation of COPD IL-8 and significantly higher remission and control group, statistically significant, suggesting that IL-8 in airway inflammation in COPD form, acute levels of IL-8 and FEV1 negatively correlated, suggesting that IL-8 in COPD airway obstruction formation.2 COPD is a combination of cytokines in chronic airway inflammation.3 acute exacerbation of COPD performance advantages for the state TH2, ease of performance advantages for the TH1 state. So before treatment increased the level of IL-4 and TH2 state and the trend is likely related to COPD patients breathe Attack...