Effect Of Huangqi On The Endogenous Nervous Stem Cell Proliferation And Differentiation In Wistar Rat Brain With EAE

Objectiveexperimental allergic encephalomyelitis(EAE) is the ideal animal model to studythe pathogenesis and therapy of acute disseminated encephalomyelitis(ADEM).According to traditional Chinese medicine theory of the brain and the spinalcord and QI and blood injury induced by EAE through detection of nervous stem cellproliferation, differentiation and expression level.Methods1 axon homogenate of cavia cobaya added to Freund adjuvant was used as antigen,Wistar rats were endermic injected through foot pads by the antigen to create EAEmodels.2 After the EAE rats were treated with Astragalus mongholicus,the proliferativeand differentiate effects of nervous stem cells were detected,specific nidogenpolyclonal antibody(neuroepithelial stem cell protein Nestin), proliferative cellmonoclonal antibody(5-bromodeoxyuridine BrdU), specific antibody of neurocyte(neuron specific enolase NSE)were used to identify proliferative faculty of nervousstem cells and its differentiated potentiality to neurons.3 EAE rats were divided to control group and treatment group randomly.neurologicdefect signs were scored at different time point, Nesin, BrdU,NSE of rat brains wereanalysed to compare expression of immuno-positive cells in dfferent groups usingimmunohistochemstry staining technique at different time points including 7 days afterintervention, 7 days, 14 days, 21 days after morbility. Results1 Neurologic defect signs score of Astragalus mongholicus treated group washigher than control group.2 Nestin and BrdU expressed unequally in different time points and differentencephalic region. Expression of immuno-positive cells of Nestin and BrdU intreatment group was significantly srronger than control group specially at 7 days and 14days after morbility, but there were no different at 7 days after intervention and 21 daysafter morbility.3 NSE expressed unequally in different time points and different encephalicregion Expression of immuno-positive cells of NSE in treatment group wassignificantly stronger than control group specially at 7 days after morbility, but therewere no difference at 7 days after intervention and 14 days ,21 days after morbility.ConclutionThe modeling of EAE is a stable and reliable method. Higher expression ofimmuno-positive cells of Nestin and BrdU in treatment group indicate nervous stemcell proliferate remarkably than control group.Higher expression of immuno-positivecell of NSE in treatment group indicate nervous stem cell differentitate remarkably thancontrol group. Treat with Astragalus mongholicus can ameliorate Nurologic defectsigns and promote the proliferation and differentiation of nervous stem cell after EAE.