Human Neural Stem Cell Transplantation For Treatment Of Experimental Autoimmune Encephalomyelitis In Rats In Experimental Studies

Objective: To explore the effect of the human neural stem cells(hNSCs) transplantation on experimental autoimmune encephalomyelitis(EAE) in rats and the status of transplanted hNSCs in the brain tissue of EAE rats.Materials and Methods: hNSCs were separated from 9-15week brains of human e mbryo,cultured and induced to differentiate. E AE rat models were established and the hNSCs were transplanted into the rat intraventricularly 10,15,21 days after immunization.The neurological signs were taken in all the groups every day after immunization. The differentiation and migration of hNSCs in vivo and the number of the demyelinated foci were determined.Results:The neural stem cells were induced to differentiate into astrocytes,oligodendrocytes and neurons in vivo. Brdu,nestn,NSE, GFAP and CNPase positive cells could be present in almost all tissue sections at every time point. Immunohistological results confirmed that the transplanted stem cells migrated to the demyelinated foci.The neural...