Expressions And Significance Of SOCS-1and C-jun In Myocardium Of Patients With Sudden Cardiac Death

Object To detect the expressions and the the relationship with sudden cardiac death.(SCD) of cytokine signaling-1(SOCS-1) and proto-oncogene proterns (C-JUN) in myocardium in patients with SCD.To investigate their correlations of SOCS-1and C-JUN, and their significance in the practice of forensic medicine.Method Forty-three cases of SCD were chosen as the experimental group while ten cases of non SCD cases as the control group. Immunohistochemical staining was employed to examine the expression of SOCS-1and C-JUN in myocardium. The SPSS18.0was used to analysis the Immunohistochemical results,and p<0.05as a statistically standards.Results1HE stainingIn the SCD group, most of them were died of coronary artery disease. And the myocardial slices of23cases had myocardial fiber fracture, myocardial interstitial edema, myocardial interstitial had a small amount of hemorrhage and lymphocytic infiltration, part of myocardial cells was hypertrophy and had fatty infiltration, eosinophilia was enhanced, part of myocardial nucleus was slightly increased, and abnormal nucleus was observed, chromosome was loose, part of myocardial cell was degenerate and necrotic, myocardial cells were slightly edema, fibrillar connective tissue increased in the myocardial interstitial. Left anterior descending coronary artery, circumflex branch and right coronary artery were noticed thickening in intimal the degree of luminal stenosis was>50%, part of the lumen occluded, part of lumen was thrombosed.2. Immunohistochemical staining①The positive expression of SOCS-1was detected in the cytoplasm in each case of SCD group. the SOCS-1distribute in the cytoplasm, tan particles was in the positive cells. The nuclei、cell membranes and interstitial had no obvious dyeing. The expression of SOCS-1in the group of SCD was significantly higher than in the negative control group(q=9.956～20.669, P<0.01)。②The positive expression of C-JUN was detected in the nucleus in each case of SCD group. The C-JUN distribute in the nucleus, tan particles was in the positive cells. The expression of C-JUN in the group of SCD was significantly higher than in the negative control group(q=4.8150～8.8832, P<0.01).③The expressions of SOCS-1and C-JUN in myocardium of patients with SCD was positively correlated(r=0.316, P=0.021).Conclusion1. The expressions of SOCS-1was increased significantly in the patients of SCD maybe invovel in the pathaphysiology of anti-apoptosisi of the myocardial cells.2. The expression of C-JUN was increased significantly in acute myocardium ischemia early in the patients of SCD, it is suggested that C-JUN may be involved in the pathogenesis of SCD. It may be become the pathology morphology auxiliary indexes to diagnosis the sudden cardiac death early,fast and sensitively.3. The increased expressions of SOCS-1and C-JUN are correlated with the incidence of SCD to some extent, they may play antagonism action in the process of SCD pathological changes, and participate in the regulation of myocardial cell apoptosis together.