| Objectives: (1) To establish a simple and reliable models of heterotopic allograft renaltransplantation in rats; (2) Investigate the value of the OPN and IL-18 expressions in theearly diagnosis of acute rat renal allograft rejection in the rat.Methods: (1) Use SD as recipients and Wistar as donors: Donor's renal artery withabdominal aorta vane was performed with end-side anastomosis to the recipient'sabdominal aorta. Donor's renal vein with inferior vena was sutured with end-sideanastomosis to the recipient's inferior vena. The rats were randomly divided into 2groups(n=25): The control group (SD-SD); The experiment group (Wistar-SD): (2) Afterrenal transplantations, 5 rats per group were killed at dayl, 3, 5, 7. The renal and bloodwere obtained. Use real-time PCR to detect the expressions of OPN and IL-18 mRNA inrenal. Use enzyme-linked immunosorbent assay (ELISA) to detect the concentration ofOPN and IL-18 in blood. The pathological character and ultrastructure of the grafts wereanalyzed by microscope and electron microscope. Meanwhile, the post operation suivivaltime of each group was recorded.Results: (1) After some modifications in rat kidney transplantations the time of operationwas reduced significantly. The total time of operation was 90-115 minutes, mean time was99.1±9.84 minutes. Among the total warm ischemia time was 1-2 seconds, cold ischemiatime was 35-40 minutes. The time of vascular anastomosis was 20-30 minutes. The successrate of renal transplantation was 88.33%. (2) The survival time of control group morethan 100 days, and the experimental group was 7.2±0.84 days. The serum creatinine of control group had no significant change during the study. The expression of OPN andIL-18 mRNA in renal also did not differ siginificantly change, so was the concentration ofOPN and IL-18 in blood. The control group kidney showed minimal histological changesafter operation. The serum creatinine of experimental group gradually elevated afteroperation (at day 7 501.40±57.53vs47.31±4.16, P＜0.01). In the experimental group, theexpressions of OPN and IL-18 mRNA increased gradualll after transplantation. In thetissue, the expression of OPN mRNA up-regulated at the day 3 and reached the high pointat day 5 (106.79±8.34vs15.67±1.36, P＜0.01). Meanwhile, the expression of IL-18 mRNAup-regulated at the day 5 and continue up-regulated till the day 7 (76.00±5.47vs6.82±0.44, P＜0.01). The experimental group kidneys at day 3, 5, 7 post-transplant revealed anextensive interstitial infiltrate consistent with severe acute rejection.Conclusions: (1) Our study implied using the donor's renal artery with abdominal aortavane and donor's renal vein with inferior vena we can perform the rat kidneytransplantation with a high success rate. (2) The Wistar-SD renal transplantation is a highrate of acute rejection and could be a model for studying the acute rejection of rat renaltransplantation. (3) OPN and IL-18 both up-regulated in acute renal allgraft transplantationin rats, and OPN up-regulates earlier. (4) The expressions of OPN and IL-18 had the samechange in blood compared with renal, and we can use non-invasible methods to detectthem. (5) OPN and IL-18 both could be sensitive factors in early diagnosis of acuterejection in rat renal transplantation, and OPN may be better.
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