Studies On Extraction And Purification Of Chlorogenic Acid In Eucommia Leaf

Eucommia(Eucommia ulmoides Oliver) is deciduous tree endemic in China, asChina's second-class protected plants. In China, Eucommia is very rich in res-ources, mainly in the south of the Qinling Mountain, Hunan, Yunnan, Guizhou,Shaanxi and other provinces. Effective components of chlorogenic acid significant-ly Detoxification, anti-bacterial and anti-inflammatory, protective and cholere- ticand enhance immunity, have good prospects for development. In recent years, Thestudy focused on the domestic Eucommia bark, as well as research on the qualityand chemical composition of crude extraction and separation of chlorogenicacid, The majority of our manufacturers to produce only very little added value,and the purity of between 20 to 50% chlorogenic acid Eucommia. Chlorogenic acid,many problems still exist and high-purity, high yield production costs low.Therefore, Eucommia leaf extract, chlorogenic acid separation technology was ofhigh purity, and has attracted people's attention.Eucommia extract from the paper on the separation of chlorogenic acid, theresults are as follows:1. In the literature based on the liquid chromatographic determination ofchlorogenic acid.HPLC conditions: column: KromasiLC18(250×4.6mm. 5μm); Thedetection wavelength: 328nm; mobile phase: methanol: water:=75:25:0.4phosphate begins: 1ml·GC column: 30℃. The method is sensitive, accurate andsuitable for the determination of chlorogenic acid.2. On the stability of the chlorogenic acid, chlorogenic acid at 60℃. Theresults showed that the aggregate 4～6 hours more stable. In alkaline conditionsdecompose easily, the most stable in the pH of acidic conditions, chlorogenic acidin the direction of such lights unstable, Chlorogenic acid in the extraction andseparation should keep acidic, dark conditions. 3. The comparative study of the extraction solvent extraction method, andfound that water extraction solvent extraction and better heating. After orthogonaltest of temperature, time, the ratio of solid to liquid and the particle size 4, theoptimization of heat extraction. The results show that: temperature 65℃, 60minutes from time, the ratio of 1:25, good particle size of 40 at present. Extractionof high and stable, and easy to operate.4. Chlorogenic acid column chromatography to determine the optimumconditions. By adsorbent eluting agent for a comparative study results showedKLFC-150, resin adsorbentTo extract the optimal conditions for ethanol, thismethod of separation of chlorogenic acid, a higher purity and yield.56.67% purityand yield of 78.3%.5. Chlorogenic acid crystallization using crude methods to furtherpurified. The crystallization of chlorogenic acid reagents and optimization ofcrystallization conditions, the optimal conditions for crystallization of chlorogenicacid: temperature of 4℃and pH value of the crystallization reagents for thePA. Through the purity of chlorogenic acid in this condition can be more than 95%.