Comparative Research On The Antioxidant And Antibacterial Properties Of Flavonoids Of Euphorbia Maculata And Euphorbia Humifusae

Chinese tridational madinice Euphorbiae spp. is the mixture of E. maculata andE. humifusae which are the annual herbs. Euphorbiae spp. mainly contains quercetinand kaempferol flavonoid compounds which have anti-bacterial, anti-virus, anti-oxidation, protecting liver, stoping bleeding and so on the biological activity.Euphorbiae spp. in our country distributes extremely widespreadly and the volume ofstore is very large, has widespread application prospect, so domestic and foreign toEuphorbiae spp. research is becoming more and deep. But E. maculata and E.humifusae which one has the more medicinal value until now not to see any researchreport. The thesis has taken E. maculata and E. humifusae as object of study,extracted active constituent in E. maculata and E. humifusae of 3 different vegetalperiods, compared with the content of total flavone and main functionality flavones,antioxidantactivity and bacteriostasis effect. Mainly obtained the following results:1. Compared with contents of total flavonoid in E. maculata and E. humifusae indifferent periods, The result indicated that (1)The contents of total flavonoid in E.maculata and E. humifusae are the highest in July, E. maculate is 20.28～22.23mg·g^-1and E. humifusae is 34.67～37.50 mg·g^-1;(2)The content of total flavonoid of E.maculate is obviously lower than E. humifusae each period.2. Mensured and compared with the contents ofquercetin and kaempferol in E.maculata and E. humifusae by using high-performance liquid chromatography(HPLC).The results suggested that: (1)The contents of quercetin and kaempferol in E.maculata and E. humifusae are the highest in July, the contents of quercetin andkaempferol in E. maculate respectively is 1.66 mg·g^-1 and 0.78 mg·g^-1; E. humifusaerespectively is 2.18 mg·g^-1 and 0.87 mg·g^-1; (2) The contents of quercetin andkaempferol of E. maculate is obviously lower than E. humifusae each period.3. Studied on reducing ability, superoxide anion and hydroxy-free radicalelimination ability, per-oxidation in liposome inhibiting ability of extraction solutionin E. maculata and E. humifusae considering vitamin C as control. The resultsindicated that, (1)The reducing ability of E. maculate is obviously weaker than E. humifusae and assumes the good proportion relations with the content of totalflavonoid;(2)The differences is not big between superoxide anion and hydroxy-freeradical elimination ability, when concentration of extraction solution is 1 mg·L^-1,superoxide anion clearance rate of E. maculata is 54% and E. humifusae is 56%;when concentration of extraction solution is 10 mg·L^-1, hydroxy-free radicalelimination ability of E. maculata is 89% and E. humifusae is 92%; whenconcentration of extraction solution is 10 g·L^-1,. per-oxidation in liposome inhibitingability of E. maculata is 66% and E. humifusae is 63%.4. Selected and used five kinds of bacteria and mild which easily make grain andfeed poisonous and mildewed, studied on bacteriostasis and sterilization effects ofextraction solution in E. maculata and E. humifusae in different periods. The resultssuggested that (1) The bacteriostast circles of bacterium or mold in E. maculata and E.humifusae from big to small are: the stage before bloom in July＞fruit stage inSeptember＞nutrition growth stage in May;(2)The order of bacteriostasis effect of E.maculate to five kinds of bacteria and mild is A. flavus＞A. niger＞S. aureus＞E. coli＞B. subtilis; E. humifusae is S. aureus＞A. flavus＞A. niger＞E. coli＞B. subtilis;(3)Thebacteriostasis effect to bacteria of E. maculate is weaker than E. humifusae; thedifferences of bacteriostasis effect to mild is not remarkable between E. maculata andE. humifusae, E. maculate is slightly stronger than E. humifusae.