Clinical Significance Of Centrosome Abnormality In Urinary Cast-Off Cells From Bladder Transitional Cell Carcinoma Suffers

Objective:To detect centrosomal abnormalities in urinary cast-off cells from bladder transitional cell carcinoma suffers,to evaluate the applicational value of centrosome abnormality detection in early and non-invasive diagnosis of bladder transitional cell carcinoma,and the relationship between centrosome abnormality and the pathological grading and clinical staging of bladder transitional cell carcinoma.Methods:Urine specimens from 35 patients with baldder transitional cell carcinoma were obtained as the tumor group,10 urine specimens from patients without a history of urothelial carcinoma were used as controls.After centrifugalization,cytospins from the urine specimens were prepared,indirect immunofluorescence staining was done to detect cellular centrosomes,the percentage of cases positive for centrosome abnormality and the percentage of cells with abnormal centrosomes were calculated,centrosome abnormality and its relationship with the pathological grades and clinical stages of bladder transitional cell carcinoma were analysed;meanwhile,DNA content of cells from these urine specimens were measured by FCM as the contrast in order to evaluate the applicational value of centrosome abnormality detection in diagnosis of bladder transitional cell carcinoma.Results:74.29% of all cases(26/35) in the tumor group were positive for centrosome abnormality,while none in the control group showed abnormal centrosomes.As for the percentage of cases positive for centrosome abnormality, there were no significant differences between grade G1,G2,G3 and between the superficial tumor(stage Tis-T1) and the invasive tumor(stage T2-T4) (P>0.05),but there were significant differences in the percentage of cells with abnormal centrosomes compared between grade G1,G2,G3 and between the superficial tumor(stage Tis-T1) and the invasive tumor(stage T2-T4) (P<0.05),the poorer the cell differentiated,the higher the percentage of cells with abnormal centrosomes was,which positively correlated with cancer grade(rs=0.76,P<0.01).On the other hand,60%of all the tumor specimens(21/35)were heteroploid measured by FCM,while 0% in the control group. The percentage of cases positive for centrosome abnormality was higher than that of heteroploid cases in anyone of the cancer grade or stage group,but there was no significant difference(P>0.05).Conclusion:Centrosome abnormality was an important event in the carcinogenesis and developing of bladder transitional cell carcinoma,detection of the centrosome abnormality was a promising approach for early and non-invasive diagnosis of bladder transitional cell carcinoma like DNA ploidy measurement with FCM.