Study On The Expression Of DNA Ploidy And Heparanase1 Gene In Tissue Of Patients With Pancreatic Tumor By Flow Cytomety

Objective Primary pancreatic carcinoma as a common malignant tumor in alimentary is a clinical symptom to hide,it make prognosis is very bad and the existence rate of PPC is very low. Its biology characteristics :easily invasion nerve and blood vessel,the rate of lymphometastasis is high,and the anatomy position of PPC is deep,and lack the particularity tumor marker and the clinical manifestations in early grade,etc. At the same time,the postoperative recurrence or metastasis of PPC is easy occurence.75% of the accurate clinical diagnosis of PPC or more is already belong to the medium or later period,the surgical operation excision rate of PPC is low, and its not sensitive to the chemotherapy and radiotherapy. The existence rate of 5 years is still 5%.The normal cell of human is changeless DNA ploidy or tetraploid. When it stimulated by cancerogenic factors , the quality and quantum of DNA will be changed, and the cell will be changed into tumorous cell which the quality and quantum of DNA was little or more than normal cells, we name the cell of DNA aneuploid or heterploid cell. It has been approved by many scientists in the world, if the quantum of DNA changed weekly, it maybe result in a malignant tumor. So it is important for diagnose a pancreatic tumor that measure and analyze cell's DNA ploid. Cancer metastasis is established by multistep tumor cell host interactions. (1) For metastasis to develop tumor cells must pass through the extracellular matrixes(ECM) and vascular basement membranes (BM).(2) Tumor angiogenesis. ECM and BM consist of large matrix molecule. To degrade these barriers, an extracellular matrix degradative enzyme is requisite. Heparanase, which is an endo-beta-Dglucuronidase, degrades heparan sulfate (HS) and heparan sulfate proteoglycans (HSPG), which localize in the extracellular matrix and on the external surface of cell membranes. Therefore, heparanase may be important in cancer invasion and metastasis of cancer. Previous studies have demonstrated heparanase expression in various malignancies and that there is differential heparanase expression between benign and malignant tumor.We checked DNA index and the tumor cell's proliferation of pancreatic tumor tissues by flow cytometry, at the same time we checked its expression of Heparanase by flow cytometry, in order to study DNA quantum,the expression of Heparanase and the pertinence DNA and Heparanase in pancreatic cancer. We expect to offer a academic and experimental gist of clinical and biological research,early diagnostic,gene cure and estimate prognosis for pancreatic tumor.Method We checked DNA quantum and proliferous performance by flow cytometry, at the same time we checked its expression of pancreatic tumor tissue and normal pancreatic tissue in its slice up of olefin by flow cytometry.Results The expression of Heparanase1 gene was associated with lymph node metastasis,TNM grade and hisological differentiation ( P< 0.05),But no relationship with age, gender and tumor size. The DNA anueploidy rate was no significantly correlated with age, gender,hisological differentiation and tumor size, and was significantly correlated with TNM stage and LN metastasis ( P< 0.05) . The SPF of pancreatic tumor was no significantly correlated with age, gender and tumor size, and was significantly correlated with TNM stage,hisological differentiation and LN metastasis ( P< 0.05) .The PI of pancreatic tumor was no significantly correlated with age and gender, and was significantly correlated with tumor size ,TNM stage,hisological differentiation and LN metastasis ( P< 0.05) .Conclusions 1.The analysis of DNA Ploidy and PI in pancreatic cancer patients can availably realize the instance of the cell multiplication and the degree of cancerous, and are closely related to their clinical biological behaviors. 2. The expression of Heparanase1 gene has significantly higher relationship with the TNM stage,hisological differentiation and LN metastasis,and maybe is a new butt for pancreatic cancer's comedy .3. The overexpression of Heparanase1 gene, aneuploid and high PI in pancreatic tumor correlate with poor hisological differentiation and metastasis.