The Expression Of DNA-PK In Esophageal Squamous Cell Carcinomas And Its Clinical Significance

Objective To detect the expression of DNA-PK: DNA-Pkcs, Ku70 and Ku80 in esophageal squamous cell carcinoma and evaluate their roles in esophageal squamous cell carcinoma with its clinicopathological features.Methods Immunohistochemical method was used to detect the expression 0f DNA-Pkcs, Ku70 and Ku80 in 52 esophageal squamous cell carcinomas, normal esophageal tissues and pericarcinoma tissues from the same patients.The result of its expression was compared in different clinicopathological features. Chi-square test and Fisher's exact probabilities were used for statistic analysis.Result DNA-PKcs expressed in all the esophageal tissues, but its expression in esophageal squamous cell carcinoma was significantly higher than that in normal esophageal tissues and pericarcinoma tissues from the same patient, and there was no statistically significant association between its expression in normal esophagus tissues and pericarcinoma tissues. Ku70/Ku80 also expressed in all the esophageal tissues, but there was no statistically significant association in esophageal carcinomas, normal esophageal tissues and pericarcinoma tissues. The positive rate of expression of DNA-PKcs and Ku70/Ku80 in esophageal squamous cell carcinoma was 84.6%, 67.3% and 65.3%; the expression of DNA-PKcs was significantly higher than that of Ku70/Ku80, there was no statistically significant association between expressions of Ku70 and Ku80. The expression of DNA-PKcs in well-differentiated carcinomas was higher than that in moderately differentiated and poorly differentiated carcinomas, but its expression had no correlation with pTNM stages and lymph node metastasis.Conclusion DNA-PKcs, Ku70 and Ku80 express in all the esophageal tissues, this demonstrates that the ability of DSBs repair as a basic function play roles not only in normal tissues but also in malignant esophageal tissues. The imbalance of expression between DNA-PKcs and Ku70/Ku80 in esophageal carcinomas may depress DSBs repair, and result in the formation of tumor. DNA-PKcs express highly in esophageal squamous cell carcinomas, it can be recognized as a new biomarker for esophageal squamous cell carcinomas different with normal tissues. DNA-PKcs express highly in well-differentiated carcinomas, and it could be predicted the degree of differentiation.