Development And Hypoglycemic Effect Of Compound Acidophilous Milk For Diabetic

OBJECTIVE:Diabetes mellitus(DM) was a endocrine metabolic disease and severe harm the health of body. The attack rate and death rate of DM increased more and more quickly. At present there were 1.5 hundred million diabetic in the world .It was more than 40000 thousand diabetic in china. Because of the decrease of islet cell and action of islet cell ,it caused the disorder of fat ,protein, water and electrolyte and produced severe complication ,for example, coronary heart disease,cerebrovascular disease,peripheral vascular disease,retinopathy and renal vascular disease ect. More than fifty percent of diabetic who succumbed to coronary heart disease. Therefore, it was very important significance for us to improve the health status of diabetic and enhence the quality of life of diabetic and prevent the complication of diabetic and promote the rehabilitation of diabetic. So the ideal method of searching for treating and controlling diabetic was very important topic of medical science boundary. So in this study we observed and researched the protective effects of Compound acidophilous milk for diabetic on Alloxan induced pancreatic gland damage and glycometabolism in rats and cut down the influence of pancreatic tissue histomorphology and lipid peroxidation and pancreatic island B apoptotic .We also Attempted to provid some significant experimental based for developing and exploiting efficiency food.METHODS:120 Adult Wistar rats(190±10) were randomly divided into Control group ,DM group ,therapy group and protective therapy group ,30 in each group. Each group were fed a basic diet and Control group were intragastric administration with Sodium Chloride, DM group and therapy group were intragastric administration with ordinary acidophilous milk, protective therapy group were intragastric administration with compound acidophilous milk of diabetic .Each guoup was intragastric administration every other day. After 15 days, the rats of DM group, therapy group and protective therapy group have been given a single shot of alloxan (50mg per 1 kg), while Control group have been given a single shot of Sodium Chloride (50mg per 1 kg) . After 5 days , determining fasting 4 hours blood sugar,then the rats of blood sugar more than 13.8 mmol/L were diabetic model rats. After the diabetic model have been induced by alloxan , the rats of therapy group were intragastric administration with compound acidophilous milk of diabetic ,and other groups have not been changed . In 30 th days and 90th days after every group were given a single shot of alloxa determines fasting 4 hours blood sugar and In 6th days,32 th days and 92th days after every group were given a single shot of alloxa carbohydrate tolerance test .In 33th days and 93th days after every group were given a single shot of alloxa, randomly drawing out 10 rats in every groups that were killed and the pancreatic gland samples and blood serum were collected .Tentative index include INS ,C-P ,MDA ,SOD ,Cr ,BUN ,TG ,TC , LDL in blood serum and GLU, glycosylated hemoglobin in blood plasm and so on.The pancreatic gland morphological structure was also obserced under lingt microscope .The degree of apoptosis was assessed with the terminal deoxynucleotidy1 transferase (TdT)-mediated d UTP nick end labeling (TUNEL) by using an in situ Apoptosis Detection Kit.The expression of INS in the cytoplasm of B cell and GLU in the cytoplasm of A cell was detected by immunohistochemistry.RESULTS:1.Effect of compound acidophilous milk for diabetic on blood-fasting sugar and sugar tolerance and glycosylated hemoglobin1.1 evel of FBS(mmol/L):1.1.1 th days after every group were given alloxa, the level of FBS in the DM group,the therapy group and the protective therapy group increased very significantly more than control group(P<0.01), but the level of FBS in the protective therapy group is lower than the DM group,the therapy group(P<0.01);1.1.2 0th days and 90th days after every group were given alloxa, the level of FBS in the DM group,the therapy group and the protective therapy group increased very significantly more than control group(P<0.01), but the level of FBS in the therapy group and the protective therapy group is lower than the DM group (P<0.01);1.2 el of sugar tolerance(mmol/L): on 6th day,32th day,92th day , compare with Control group, the difference of sugar tolerancein 0.5h,1.0h,1.5h,2.0h of DM group , therapy group, protective therapy group is very significantly (P<0.05) while compare with DM group ,the difference of sugar tolerance in 0.5h,1.0h,1.5h,2.0h of therapy group, protective therapy group is very significantly (P<0.01). sugar tolerance of 0.5h,1.0h,1.5h,2.0h of 6th and sugar tolerance of 1.0h,1.5h of 32th of protective therapy group were different from DM group'and therapy group',and the difference were very significantly(P<0.05)(p<0.01).1.3 evel of glycosylated hemoglobin(%):93th days after every group were given alloxa, the level of glycosylated hemoglobin in the DM group,the therapy group and the protective therapy group increased very significantly more than control group(P<0.01), but the level of glycosylated hemoglobin in the therapy group and the protective therapy group is lower than the DM group (P<0.01);2.Effect of compound acidophilous milk for diabetic on pancreatic function2.1 evel of serum INS (μIU/ml):33 th days and 93th days after every group were given alloxa, the level of INS in the DM group ,the therapy group and the protective therapy group is more lower than control group(P<0.01), but the level of INS in the therapy group and the protective therapy group is very significantly higher than the DM group(P<0.05 and P<0.01);2.2 evel of serum C-P(ng/ml): 33th days and 93th days after every group were given alloxa, the level of C-P in the control group, the therapy group and the protective therapy group is very significantly higher than the DM group(P<0.01,P<0.05 and P<0.01);furthermore, the level of C-P in the therapy group and the protective therapy group doses not have significant difference.2.3 evel of plasm GLU(pg/ml): 93th days after every group were given alloxa , the Level of plasm GLU in the control group and the protective therapy group is very significantly lower than the DM group(P<0.01 and P<0.05);the Level of plasm GLU in the therapy group is higher control group ,but also significantly lower than the DM group(P<0.05).3.Effect of compound acidophilous milk for diabetic on serum SOD,MDA 33th days and 93 days after every group were given alloxa,the level of SOD in DM group, the therapy group and the protective therapy group is very significantly lower than control group(P<0.01),the level of MDA in the DM group,the therapy group and the protective therapy group is more higher than control group(P<0.01),but the level of SOD in the therapy group and the protective therap is higher than DM group(P<0.01), the level of MDA in the therapy group and the protective therapy group is very significantly lower than the DM group (p<0.05 or P<0.01);4.Effect of compound acidophilous milk for diabetic on sersum TG,TC and LDL 93 days after every group were given alloxa,the level of TG,TC and LDL in the therapy group and the protective therapy group is very significantly lower than DM group(P<0.05 and P<0.01).5.Effect of compound acidophilous milk for diabetic on sersum Cr and BUN(μmol/L) 93 days after every group were given alloxa, the level of serum Cr and BUN in the DM group ,the therapy group , the protective therapy group is very significantly higher than control group(P<0.01),but the level of serum Cr and BUN in the therapy group , the protective therapy group is very significantly lower than DM group(P<0.05,P<0.01)6.Effent of compound acidophilous milk of diabetic on the pancreatic gland morpholological structureExamination of histological structure under a light microscope indicated that pancreatic islets of control group were colored to pale colour and bouncary distinctness, morphous regulation, lineing up in order.The number of pancreatic islet and isle cells in control group is more than DM group .Cell nucleus of control group were blue and limpid. Chromatospherite is conspicuous. Pancreatic islet cellular structure of DM group disordered and morphous is inordinate. Lots of cell nucleus of DM group were deformed, pycnotic and emerged to vacuolar degeneration. The number of pancreatic islet and isle cells in the therapy group was more than DM group but lower than control group. A little of cell nucleus were deformed and pycnotic. Islet cells of the protective therapy group were closed to control group and bouncary distinctness, morphous regulation, lineing up in order.7.Express of the INS and GLU in the endochylema of pancreatic IsletExamination of immunohistochemistry under a light microscope indicated that Islet B cells of control group secretory INS were more than other groups and secreted lots of grains .Endochylema of cells were colored to deep color. Islet B cells of DM group secreted a little of grains. Endochylema of cells were colored to tint. The number and color of grains in the therapy group was more than DM group , furthermore ,the number and color of grains in the protective therapy group was closed to control group.Islet A cells of control group secretory GLU were less than other groups and the GLU grains situated In the fringe of pancreatic islet .Endochylema of cells were colored to tint color. Islet A cells of DM group secreted lots of grains that a part of have intruded into the middle of Islet. The number and color of grains in the therapy group was less than DM group , furthermore ,the number and color of grains in the protective therapy group was closed to control group.8.The alloxan-induced TUNEL-positive B cells in pancreatic islet in the DM group were particularly more than in the therapy group and protective therapy group. Further,there was only a few TUNEL-positive B cells in the control group.CONCLUSIONS:1.The result of FBS, OTGC ,HGb of the rats indicated compound acidophilous milk of diabetic could obviously improve glycometabolism and decease effect of blood sugar ;2.The observation of pancreatic island patho-structure, immunity class of pancreatic island histiocyte endochylema and the B apoptosis of pancreatic island indicated compound acidophilous milk of diabetic have protection for the structure and function of pancreatic island;3.The result of sersum MDA and SOD indicated that compound acidophilous milk of diabetic could bviously lessen oxidative damage of organism in DM rats. It may be that compound acidophilous milk of diabetic had protection for the structure and function of pancreatic island;4.The result of serumtTG, TC and LDL indicated that compound acidophilous milk of diabetic could have action of improving disorder of lipid metabolism in DM rats;5.The finding indicated compound acidophilous milk of diabetic could have the protection of lessening kidney damage of the DM, infection prevention and cutting down death rate rats;6.Beause of protective therapy group were intragastric administration with compound acidophilous milk of diabetic before founding DM model ,every index of animal experiment indicated protective therapy group had best effect .This indicated that compound acidophilous milk of diabetic had potential application prospect for preventing diabetes; 7.Compound acidophilous milk of diabetic had above-mentioned effect that because of there were lots of nutrition efftective ingredient in prescription of compound acidophilous milk of diabetic;...