The Expression Of Transforming Growth Factor β1 And Its Ⅰ,Ⅱ Type Receptors In The Development Of Rat Embryo And Heart,Liver,Lung,Kidney

Transforming growth factor beta are multifunctional cytokines, which generally regulat many bio-effections in the cell.They also play an important role in the development of organ and embryo growth. Transforming growth factor beta-1 is one subtype of this family, which has close relationship with keepingpregnancy and embryo growthing. Transforming growth factor beta-1 educes effection by binding specificity receptors on the cell surface. When it binds transforming growth factor beta I,II type receptor, they co-composit ligand-receptor tricompounds.The tricompounds is the key to implement cellular inner potency.This experiment firstly apply half-quantity RT-PCR. In orderto sure RT-PCR reaction in linear extent before stable phase, weuse detection step by step and combinat BIO-RAD Quantity Onesoftware analysising results. At last we get three factorscirfrequency of RT-PCR reaction. We repeat this experiment threetimes per-factors, finding this three factors' cirfrequency are all 28 times. We devise competitive experiment inβ-actin andpreparation amplification factors, findingβ-actin andtransforming growth factor beta-1,β-actin and transforminggrowth betaⅠtype receptor ,β-actinand transforming growthbetaⅡtype receptor amplificating satisfactory electrophoresisstraps. Base on above-mentioned theory, we use half-quantityRT-PCR detecting expression of transforming growth factor beta-1and transforming growth factor betaⅠ,Ⅱtype receptor in 13th～17th SD rat entire embryo. We find expressions of transforminggrowth factor beta-l in 13th～16th increasing gradually, among thetotal 15th,16th are expressions's peak, till 17th is expressionslow. The expressions of transforming growth factor betaⅠ,Ⅱtypereceptor are all increasing in this stage, till 17th they get peaktogether. In 15th～17th the total change of three factorsexpressions is obviously, so we choice 15t～17th asimmunohistochemical staining observation time. Then we adoptimmunohistochemical staining to observe space-time expressionsof these three factors in heart, liver, lung, kidney. Theconclusion is that the expressions of transforming growth factorbeta-1 and transforming growth factor beta I, II type receptorare obviously in 13th～17th SD rat embryonic heart and theexpressions of degree are all alike in the same time. But theexpressions of these three factors are negative in heart valve.The expressions of transforming growth factor beta-1 are negativein 13th～17th SD rat embryonic liver. But the expression of transforming growth factor betaⅠ,Ⅱtype receptor appearancedynamic changings that the former become increase and the laterbecome decrease. Transforming growth factor betaⅠtype receptorappearance most earlierly in in embryonic kidney and transforminggrowth factor beta-i, transforming growth factor betaⅡtypereceptor appearance at nearly same time. The expression oftransforming growth factor betaⅠ,Ⅱtype receptor are almostlikewise, but the desmohemoblast cell expressions oftransforming growth factor beta-1 are only in tubiform epithelialcells. The expression of transforming growth factor beta-lbecomeincrease with gestational age in 15th～17th SD rat embryonic lung,hut the expression of transforming growth factor betaⅠtypereceptor are not changings and the expressions of transforminggrowth factor betaⅡtype receptor are increased firstly thendecreased latterly. At the time, the expression of these threefactors are all increased firstly and decreased latterly inend-bud columnar epithelium of embryonic lung.In a word, the expressions of transforming growth factorbeta-1 and transforming growth factor betaⅠ,Ⅱtype receptor arepresent different phase regular pattern in different organswithdevelopment of SD embryo. We know the signal-transmit-iter abouttransforming growth factor beta make significance contribution inembryonic vegetation. But these different expression pattern leadwe to presumethese three factors may have itself biodynatron styleexcept orthodox signal-transmit-iter.