Expression Of Ephrin-B3mRNA And Change Of Apoptosis In Brain Tissues Of Neonatal Rats With PVL

Objective1.To set up the neonatal rat PVL ( Periventricular leukomalacia) model, to detect the pathological changes of brain in those with PVL ;2.To study the expression of ephrin-B3mRNA and change of apoptosis in the brain tissues of neonatal rats with PVL;3.To investigate the correlations between ephrin-B3 and apoptosis.4.To explore the functions of ephrin-B3 in mechanism during PVL and CNS regrowth inhibition.MethodsA total of 160 2-d SD rats were randomly divided into experimental group (86) and control group(74). After establishment of PVL model ,the rats were executed at Oh, 8h,24h,48h,72h, 7d after hypoxic-ischemia. HE stain and light microscope were used to observe the pathological changes of brain tissues.Real time RT-PCR method was applied to detect the changes in the expressions of ephrin-B3mRNA,DAPI was applied to detect apoptotic cells in the brain tissues. SPSS 11.0 software was used to analyze the data, a =0.05 was considered the level of significance. Results1. Observed by light microscope: In the control group, brains cells were arrayed in an orderly manner with big nucleus, less cytoplasm and symmetrical vertical on both left and right sides; while in the experimental group, the pathological changes of brain tissues included cellular swelling, pyknosis, reticular necrosis and so on in early period of hypoxic-ischemic(HI). 7d after HI, sieve-like necrosis was observed in the preventricular white matter.2. Expression of ephrin-B3mRNA : the expression of ephrin-B3mRNA in the experimental group reached the peak value at 72h, demonstrating significant differences at 8h,24h,48h,72h, 7d compared with the control group(P<0.05).3. changes of apoptosis index: AI reached the peak value at 48h, demonstrating significant differences at 8h,24h,48h,72h compared with the control group(P<0.05).Conclusions1.the pathological changes of brain tissues from the 2-d SD rats with PVL have been proved to accord with PVL by the observation under light microscope ,and also the experimental group expressed lower weight and abnormal behavior,Which means that the establishment of PVL model is successful.2.In the PVL neonatal rat, ephrin-B3mRNA increased significantly compared with the control group, which suggests that ephrin-B3 may participate in the pathogenesis of PVL,it may play important functions in CNS regrowth inhibition. 3. the AI increased significantly compared with the control group, which suggests that apoptosis is the major pathogenic pattern of HI for immature rats.4.In the PVL neonatal rat,the Expression of ephrin-B3mRNA and the apoptosis index have similar tendency ,which suggests that there are some correlations between ephrin-B3 and apoptosis in the brain tissues.