| BACKGROUND:Iron deficiency anemia (IDA) is one of the highest incidencenutritional-deficiency diseases all over the world; especially infants and children arethe main group. IDA presently becomes one of the most important nutritionalproblems to be solved.OBJECTIVETo observe the effect of chocolate carrier and orange juice on recovery of IDAmodel rats.MATERIALS:The experiment was carried out in the Laboratory of Nutrient and Food Hygiene,School of Public Health And Tropiacal Medicine, Southern Medical University fromMarch to June 2006. A total of 60 healthy SD rats of clean grade were provided byAnimal Center of Southern Medical University (certification: 2002-009 2005A047).METHODS:①Establishment of IDA models: Among them, 20 rats of half genders wererandomly selected to regard as control group, and other 40 were regarded as modelgroup. Rats in control group were fed with routine feed and drank freely. Rats inmodel group were fed with AOAC-modified low-dosage iron feeds to establish IDAmodels by blooding at caudal vein. Three weeks later, average concentration offerrohemoglobin in model group was decreased to about 78g/L, and this suggested that model establishment was successful. Ten rats of half genders in each group wererandomly sacrificed. Pre-experiment and 3 weeks of post-experiment, rats wereweighed to measure concentration of ferrohemoglobin with hemoglobin cyanide(HiCN) technique, red blood cell count (RBC, direct method), serum iron(microparticle chemiluminescent immunoassay and related kit), concentration ofserum transferrin receptor (STFR, ELISA method and related kit) and free erythrocyteprotoporphyrin(FEP, fast microamount fluorometric method)②Recovery test: Other10 rats in control group were regarded as normal control group, and they were fedwith routine feed and drank freely. The rest 30 rats of half genders in model groupwere randomly divided into 3 subgroups: model control group, FeSO4 group andchocolate & orange juice group with 10 in each group. Rats in model control groupwere perfused with distilled water everyday; rats in FeSO4 group were perfused withFeSO4, and rats in chocolate & orange juice group were perfused with chocolatecarrier and orange juice. The iron volume in the last two groups was 6mg/(kg·d). At40 days after intervention, the experiment was stopped. Concentration offerrohemoglobin, RBC, serum iron, concentration of STFR and activity ofplasma-protein aconitase were measured with atom-trapping atomic-absorptionspectrophotometry; meanwhile, biological utilization rate of chocolate carrier &orange juice was calculated. The effect of iron regulatory protein and TfR in theliver regulating the recovery during IDA from molecular level.MAIN OUTCOME MEASURES:①Contents of herrohemoglobin, RBC, serum iron and STFR before experimentand after modeling and Lib-protoporphyrin of red blood cell;①contents offerrohemoglobin, RBC, serum iron, STFR and activity of plasma-protein aconitasebefore recovery test and at 40 days after experiment;③Related biological utilizationrate.④The effect of iron regulatory protein and TfR in the liver regulating therecovery during IDA.RESULTS:①Comparison of blood index after modelling: Content of ferrohemoglobin after modelling, RBC and content of serum iron were lower in model group than those inbefore modelling (P＜0.01), but content of STFR was higher than that in beforemodelling (P＜0.01).②Comparison of blood index and activity of plasma-proteinaconitase in liver at 40 days after experiment: At 40 days after intervention,concentration of ferrohemoglobin, RBC and content of serum iron were higher inFeSO4 group and chocolate & orange juice group than those in model control group(P＜0.01); however, content of STFR was lower than that in model control group(P＜0.01). At 40 days after intervention, activity of plasma-protein aconitase in FeSO4group and chocolate & orange juice group was higher than model control group(P＜0.01).③Related biological utilization rate of chocolate carrier plus orange juice:Biological utilization rate of FeSO4 was regarded as 100%, and biological utilizationrate of chocolate carrier plus orange juice was increased remarkably (106%).④Express of RP2 mRNA and TfR mRNA in the liver show both has significantassociativity.That also explain IRP2 regulate TfR mRNA after the transcription whichaffect the store of iron in liver.It is a important iron regulator in vivo.CONCLUSION:Chocolate carrier plus orange juice can improve IDA function and wildly use ontreating IDA because of its good absorption. It is characterized by well biologicalutilization rate and good taste; therefore, it is a hot topic for trophology and foodsproduce presently. |
PDF Full Text Request