Study Of Orange Leaf Quality Evaluation System

OBJECTIVE: Characteristic by using the pharmacognosy identification, determination ofmain effective component content and regular inspection and a series of detection means,in different regions in orange leaf quality evaluation of systematic and in-depth research, todevelop orange leaves the standardized quality control standards and evaluation system, toensure that the quality of safe, effective, stable and controllable.METHODS: Using character identification, microscopic identification method for the10batches of orange leaves were conventional medicine research; accordance with the2010version of "Chinese Pharmacopoeia" method, the orange leaves of herbs moisture, ash,extract to determine the content; using thin layer chromatography Orange leaf herbs forqualitative identification of hesperidin; by steam distillation of orange leaves volatile oilcontent were determined; anthrone method using sulfuric acid polysaccharide content oforange leaves were determined; investigated extract hesperidin orange leaves the methodand its determination; guidance orange leaves quality standards by experimental results.RESULTS:1Pharmacognosy Research by orange leaves of different origin, and foundmicroscopic characteristics consistent with each batch of samples: This product isyellow-green or gray-green powder. Calcium oxalate crystals common, scattered or existwithin the parenchyma cells, diameter4~10μm. Fiber bundles surrounding parenchymacells often contain calcium oxalate crystal formation crystal fibers. Catheter common,mostly ladder pattern catheter, a catheter threaded occasionally. Stomata infinitive,subsidiary cells4to6. Orange leaves TLC hesperidin significantly brighter spots in thecorresponding position. This can be qualitative identification.2contents were investigatedorange leaves moisture, ash, extract, etc, to develop orange leaves medicinal qualitystandard moisture content must not exceed8.0%of the total ash content limit is not exceed 15.0%, acid insoluble ash limit is not exceed1.5%aqueous extractives content of not lessthan30.00%, alcohol soluble extract content of not less than25.0%, for the further studyof the active ingredient to provide guidance and advice.3using sulfuric acid anthronemethod for different batches to determine the content of orange leaves, orange leaves, andpreferably a polysaccharide best extraction method, the optimum concentration of alcoholprecipitation is80%solid to liquid ratio of1:20, extraction time to40min, recommendedthe development of orange leaves polysaccharide content of not less than4%, solublesugar content of not less than10%of the total sugar content of not less than14%.4usingsteam distillation method for different batches orange leaves volatile oil content weremeasured, and preferably orange leaves optimum extraction of volatile oil, the besttechnology for the solid-liquid ratio of1:8, soak3h, extracted8h, recommended every150g Orange leaf volatile oil volume of not less than0.50mL.5Extraction Methodorange leaves hesperidin main active substance in refluxing120min better, using thismethod to extract orange leaves the determination of hesperidin, hesperidin content wasmeasured by HPLC.CONCLUSION: Through the orange leaves of different origin Pharmacognosy Researchand effective composition determination, etc, to develop a more comprehensive qualitycontrol standards and the drafting of the draft instructions, the whole system from thescientific evaluation of the quality of orange leaves.