The Research Of Molecular Biological Mechanism Of Exogenous BMP On The Local Treatment Of Postmenapausal Osteoprosis

Postmenopausal osteoporosis (PMO) is one of the common metabolic bone diseases in postmenopausal women. Estrogen hormone deficiency has long been known to result in abnormal of bone metabolism in postmenopausal. Although estrogen replacement therapy has been widely used to prevent and treat PMO, adverse effects of this method on patient's health limit its application for PMO. Bone Morphogenetic Protein (BMP) has strong activity of osteoinduction, and was used successfully in treating bone nonunion and bone defection, but basic research is needed for whether BMP could be used in preventing and treating postmenopausal osteoporosis and fracture.With an attempt to investigate the molecular biological mechanism of exogenous BMP in preventing and treating PMO, the following researches were carried out: 1) female goats were ovariectomized to established the animal model of PMO. Normal sodium(NS),fibrin sealant(FS) and fibrin sealant + BMP were injected respectively into distal radials of goats. 2) the BMD of distal radials was measured by using Micro-CT. The expression of IGF-1,OPG and TNF-αwas detected by immunohistochemisty,hybridization in situ and image analysis.The results revealed that: 1) 5 weeks after injection , BMD detected by Micro-CT of FS+BMP group increased more significantly than that of NS and FS group (P <0.05), and no significant difference of BMD was found between NS group and FS group. 2) 5 weeks after injection , the gray-scale of IGF-I and OPG of cancellous bone in FS+BMP was significantly lower than in NS and FS group(P<0.01), and the grayscale of TNF-αof cancellous bone in FS+BMP was higher than that in NS and FS group (P<0.01) ,and no significant difference of grayscale of IGF-I,OPG and TNF-αwas found between NS group and FS group.Conclution:exogenous BMP on the local treatment can protect local BMD and microstructure during lower lever of the contents of estradiol; exogenous BMP has antiosteoporotic effects on ovariectomized goats and the mechanism was realized by up-regulation of expression of IGF-1 and OPG and down-regulation of expression of FNF-αto promot bone formation and inhibit bone absorption probably.