The Research On Recovery Of Cavernous Nerves Injury Through Transcetaneous Electrical Stimulation In Rat

[Background] Penile erectile dysfunction is the most common male sexual dysfunction. Clinical forensic medicine refers to penile erection identification because of physical injury, traffic accident, divorce, rape, medical tangle, etc. Although with the help of nocturnal penile tumescence, penile color Doppler ultrasonography, pudendum evoked potential, etc. we can identify the penile erectile function and distinguish the relationship between erectile function and injury. To make a further study, investigate the diagnostic criteria of erectile dysfunction in molecular level, animal model must be established. As the most important nerve which dominate penile erection, cavernous nerve is the first choice to establish the model of neurologic erectile dysfunction. While rat has much similarity in blood vessel and neuro-structure of pelvic cavity and penile with human being , it's price is optimal animal, so cavernous nerves injury of rat is the ideal animal for the model of neurologic erectile dysfunction. With the development of medical science, diagnostic technique of neurologic erectile dysfunction has gained great progress, while the treatment of neurologic erectile dysfunction is still difficult, until now there is no effective method. As a traditional rehabilitation therapy of nerves, electric stimulation may promote nerve regeneration by promoting growth factor chemotaxis and increasing Schwann cell activity. Nitric oxide is the key neurotransmitter in penile erection which relaxes smooth muscle cell of corpus cavernosum resulting in erection. Nitric oxide is generated from arginine by the action of nitric oxide synthase. The determination of neuronal nitric oxide synthase(nNOS) can reflect condition of cavernous nerves recovery, reflect the erectile function recovery indirectly.【object】We established the model of neurologic erectile dysfunction by nipping bilateral cavernous nerves in rats. And researched the recovery of cavernous nerves and changes of nNOS through timed and quantitative transcetaneous electrical stimulation. Our study offered an essential foundation for further study on erectile dysfunction, at the same time provided a simple but feasible method for erectile dysfunction treatment.【Method】30 male Sprague Dawley rats were randomized into injury group (20) and control group (10), bilateral cavernous nerves were nipped in injury group to establish the model of neurologic erectile dysfunction. Another 30 Sprague Dawley male rats were randomized into injury group (20) and control group (10), among injury group only 10 rats were given transcetaneous electrical stimulation. One month after surgery, nNOS-containing nerve fibers in corpus cavernosum of rats were counted to evaluate the model of injury and the recovery after electrical simulation.【Result】Observe four high power fields randomly, count grey scale and absorbance of nNOS positive neurones . normal control group were 180.39±3.77 and 8.25±1.03, injury group without transcetaneous electrical stimulation were 198.12±3.27 and 6.12±1.17, injury group with transcetaneous electrical stimulation were 191.27±4.20 and 7.68±1.72. Significant difference existed in the grey scale and absorbance of nNOS positive neurones between injury group and control group (P<0.05). Significant difference also existed in the grey scale and absorbance of nNOS positive neurones between the groups whether giving electrical simulation or not (P<0.05).[Conclusions] The major pelvic ganglion lies on either side of the dorsolateral lobes of the prostate. The largest outflow is termed the cavernous nerve. Bilateral cavernous nerves nipped can cause erectile dysfunction. It is a well method to establish the model of neurologic erectile dysfunction by Nipping bilateral cavernous nerves in rats. Timed and quantitative transcetaneous electrical stimulation can promote the recovery of cavernous nerves through the change of nNOS. Consider the condition that a great quantity of neurologic erectile dysfunction caused by cavernous nerves injury Our study offer an essential foundation for further study on erectile dysfunction, at the same time provide a simple but feasible method for ED treatment.