| Objective: On dialysis solution of ascorbic acid can reduce the hemodialysis patients with vascular endothelial cell injury.Methods: Experimental group: 1. Uremic not dialysis group of 10 patients (serum creatinine> 707μmom/L, urea nitrogen>28.6 mom/ L). Control group of 15 cases. 3. Treatment group of 30 patients the control group and treatment group I choose conventional dialysis center hemodialysis for more than six months,dialysis three times a week, four hours each time,both the conventional hemodialysis way. control group conventional dialysis fluid composition formula three months of hemodialysis treatment group with the use of fresh-prepared bad blood acid 2 g / L concentration of acid dialysis solution (A solution) on hemodialysis for three months. Uremic not mining blood dialysis group, the treatment group and the control group respectively before and after treatment three months before mining hemodialysis blood. A "Percoll density gradient centrifugation method" factorⅧassociated antigen (Ⅷ- Rg) immunofluorescence assay number of CEC. RIA detection of IL-6 and TNFα; double-antibody sandwich ELISA for detection of ET-1 andTM; epidemic scattering turbidimetric method for detection of SCRP. Application of the results of SAS statistical software for analysis, comparison group using t-test and rank sum test, P <0.05 for the difference is significant, the correlation between the parameters of using Pearson's correlation analysis.Results: Experiment control group and the treatment group CEC, TNFα, IL-6, S-CRP, ET-1, TM compared with the level of uremic not dialysis group increased (P> 0.05). Group and the control group without treatment significant changes (P <0.05). Uremic group of dialysis before and after the experiment CEC,TNFα,IL-6, S-CRP, ET-1, TM level no significant change in the control group after experiment CEC, TNFα, IL-6, S-CRP, ET-1, TM level higher than the pre-test (P> 0.05). Experimental group in the experiment CEC, TNFα, IL-6, S-CRP, ET-1, TM The lower level than before the experiment (P> 0.05).Discussion: End-stage renal failure patients rely mainly on the replacement therapy and survival, hemodialysis is the most commonly used one of the ways, but its impact on the cardiovascular complications of ESRD is the major factor in the survival of patients. Study of vascular endothelial injury is triggered and ( or) to promote atherosclerosis, hypertension,myocardial infarction, congestive heart failure and the key factor, and endothelial cells generally considered to be obstacles to dialysis and non-dialysis patients with uremic the basic characteristics. Vascular endothelial cells in the blood vessel lining is not just a simple barrier layer, but also the body's largest organ endocrine and paracrine secretion through the production and release of various substances regulation of vascular function,thrombosis, vascular tone regulation and immune response ,and other important aspects of the biological significance. When endothelial cell damage or activation, and its barrier function will change. Hemodialysis, some of its blood in vitro cycles inevitably lead to hemodynamic changes, access channels, and for dialysis membrane and blood compatibility, and so could lead to cells Components different levels of activated endothelial cell layer eventually caused structural and functional changes. Maintenance hemodialysis patients with renal dysfunction because of their long-term dialysis, and the role of exogenous nature as a result of oxidation of the material increased the level of absolute or relative, and the oxidation reaction of factors involved in a variety of end products pathophysiological changes and endothelial cell injury and changes in the permeability of endothelial cells and leukocyte adhesion ability. study showed that reactive oxygen species in the first attack on the role is to play defense ascorbic acid, ascorbic acid and vitamin E in the regeneration and reduction activity plays a key role in maintaining. Uremia patient study found that plasma ascorbic acid levels were significantly lower, but also in the process of further decline in hemodialysis Based on the above situation, the application of ascorbic acid dialysis fluid through the pump can be added to ascorbic acid total plasma ascorbic acid stability in a relatively high level,and dialysis fluid side of the ascorbic acid in the environment does not enter the body directly under the premise of blood in the membrane interface rapid reaction play its antioxidant role of free radicals. passage of this We study found:uremia not in the experimental group before and after hemodialysis vascular endothelial cell injury markers no significant changes in the control group patients in the experimental vascular endothelial cells after injury showed signs of an increase in the experiment, and experiments in the treatment group after its above-mentioned indicators decline,we think that ascorbic acid dialysis solution hemodialysis patients may improve vascular endothelial cell injury.Conclusion: Hemodialysis patients with vascular endothelial cell injury may be more serious uremia of dialysis patients. Hemodialysis patients may increase the vascular endothelial cell injury. Ascorbic acid dialysis solution hemodialysis patients may improve vascular endothelial cell injury... |
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