| Aim: to set up quantitative measure and qualitative specific fingerprint spectrum of Angong Niuhuang WAN by TLC. Method: to differentiate qualitatively the main gradients Calculus Bovis. Result: Calculus Bovis can be detected dramatically by TLC. The paper adopt TLCS method to determine the content of cholic acid in Angong Niuhuang Wan by taking silicagel G as thin layer absorbent; chloroform-Ether-glacial acetic acid as developer (2:2:1); 10% phosphorus molybbic acid alcohol solution as coloration. According to Linear scanning by TLCS; detecter is 700nm, reference is 450nm; r = 0.9985, linear limits: 0.768~2.304μg; average reclaim proportion: 97.3%, RSD=2.60%,n=5. Conclusion: the characteristic finger-print spetrum is a rapid and accurate method for quantitative and qualitative measurement for internal control of the preparation.
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