| Glioma, a kind of malignant tumor in central nervous system, has a high disease rate which is above 40 percent of all kinds of nervous system tumor. To malignant glioma, we presently choose the operation to remove the tumor, and assist with radiotherapy and chemotherapy after operation. Though combined therapy have extended the average suivival time for maglinant glioma patients, the prognosis is not ideal. Recently,Molecular targeting in the treatment of cancer is nowadays becoming a promising approach based on research progress on pathogenesis and molecular mechanism of glioma. The new oncology strategies of molecular targeting became the highlight of promising biotherapy/immunotherapy in the treatment of cancer.RNA interference (RNAi) is a recently discovered, powerful molecular mechanism that can be harnessed to engineer gene-specific silencing in mammalian tissues.RNA interference(RNi) is widespread natural phenomenon.RNAi refers to Phenomenon that endogenous or exogenous double-stranded RNA(dsRNA) mediate Degradation of specific mRNA,which result in silence of target genes and bring about Functional absent phenotype.RNi regulates gene expression after transcription.It has very important effect in the area of study gene function,gene knock out,adjustment of gene expression and gene therapy.The average quantity of PTTG mRNA was detected in a significantly greater proportion in high pathologic grades.The expression of PTTG was related to MVD ,PCNA,bFGF,but wa irrelevant to sex,age,position of tumor and diameter of tumor.PTTG is involved in the early stage of tumorigenesis of glioma.It was a factor in tumor angiogenesis and proliferation.Objective:The study was desinged to silence PTTG expression in U251 human glioma cell line by siRNA tagreting PTTG to investigate the effects of PTTG sileneing on proliferation, cell cycle control,cell apoptosis, invasive abilities and cell migration ability of U251 human glioma cell.And then to elucidate the feasibility thatMethod:1. The siRNA against PTTG was delivered by Lipo2000+ pGenesil2-PTTG siRNA , Lipo2000+HK carrying a scrambled fragment as siRNA negative control,and untreated U251 cell as negative contral.2. Cell proliferative were assessed by MTT assay.3. Flow cytometry and PI-staining was performed to analyze cell cycle and cell apoptosis percentage .4. Tumor cell invasive abilities were examined by Transwell/Matrigel method.5. Cell migration abilities were assessed by wound-healing method.Result:The Cellular proliferation and growth were inhibited significantly.Cell apoptosis percentage was increased .The invasive activity and cell migration abilit was dropped down in pGenesil2- PTTG siRNA vector transfected cells as compared with parental and HK vector transfected cells.Conclusion:1. RNA interference targeting PTTG gene may control cell line U251 cell cycle,inhibit of tumor cell proliferation,promote apoptosis of tumor cells, weaken tumor cell invasive abilities and migration abilities;2. PTTG plays an important role in cell proliferation, apoptosis, migration and invasion of human brain glioma cells, which may be a promising target for tumor gene therapy.3.RNA interference technology will be one of the Glioma new means of gene therapy.
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