Experimental Studies On The Cellular Affinity Of The Bionic Materials-the Inorganic Active Element Induced Bone Scaffold Materials

Objective: The inorganic active element induced bone scaffold materials were used as artificial extracellular matrix, BMSCs were used as seed cells. According to the physical measurement on bionic characteristics about this materials, the cellular affinity of it was evaluated, which provided theoretical and experimental basis for the inorganic active element induced bone scaffold materials.Methods: The specific surface area , pore size distribution, porous ratio, permeability ratio of the inorganic active element induced bone scaffold materials were evaluated in vitro. BMSCs were cultured and identified by the flow cytometry ,the third passage of it was used to culture with scaffold materials, to compare with the cells cultured with pure fetal calf serum as the control group. MTT assay was used to examine the effects of scaffold materials on BMSCs viability, the growth of cells was observed by scanning electron microscope(SEM).Results: The specific surface area of the inorganic active element induced bone scaffold materials was 210m~2/g, mean pore size was 6nm, porous ratio was 90%, permeability ratio was 34%. MTT assay showed significant difference in the number of cells between the scaffold materials and the control group(P<0.05)on the seventh day, BMSCs co-cultured with scaffold materials exhibited good growth as observed under SEM.Conclusions :The inorganic active element induced bone scaffold materials possess preferable cellular affinity, which could be the high potential scaffold material of bone tissue engineering.